AlanFung:LabNotes/Capturing/2014-1-23: Difference between revisions
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>Alan6017518 (Created page with "==Capturing ppMALAT1 template w ppMALT1 ordered form IDT== ==Probe calculations ppMALAT1==") |
>Alan6017518 |
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==Capturing ppMALAT1 template w ppMALT1 ordered form IDT== | ==Capturing ppMALAT1 template w ppMALT1 ordered form IDT== | ||
==TBU GEL to verify probe template size== | |||
*Aim for 100ng/band | |||
*10bp Ladder | |||
*ppMALAT1 (92nt, 100uM=3036ng/ul) | |||
*MALAT1 Template (45nt, 100uM=1485ng/ul) | |||
*ppillumina_155 (99nt, 100uM=3267ng/ul) | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext> | |||
|- align="center" | |||
| width="274" height="13" | Sample | |||
| width="75" | ng/ul | |||
|style="background-color:#EBF1DE" width="75" | ul | |||
| width="273" | ng/ul | |||
| width="147" | ul | |||
|style="background-color:#EBF1DE" width="75" | H2O | |||
|- align="center" | |||
| height="13" | ppMALAT1 | |||
| align="center" | 3036 | |||
|style="background-color:#EBF1DE" align="center" | 0.99 | |||
| align="center" | 100 | |||
| align="center" | 30 | |||
|style="background-color:#EBF1DE" align="center" | 29.01 | |||
|- align="center" | |||
| height="13" | MALAT1 Template | |||
| align="center" | 1485 | |||
|style="background-color:#EBF1DE" align="center" | 1.35 | |||
| align="center" | 100 | |||
| align="center" | 20 | |||
|style="background-color:#EBF1DE" align="center" | 18.65 | |||
|- align="center" | |||
| height="13" | ppillumina_155 | |||
| align="center" | 3267 | |||
|style="background-color:#EBF1DE" align="center" | 0.92 | |||
| align="center" | 100 | |||
| align="center" | 30 | |||
|style="background-color:#EBF1DE" align="center" | 29.08 | |||
|- align="center" | |||
| height="13" | 10BP | |||
| align="center" | 1000 | |||
|style="background-color:#EBF1DE" align="center" | 0.10 | |||
| align="center" | 100 | |||
| align="center" | 1 | |||
|style="background-color:#EBF1DE" align="center" | 99 | |||
|} | |||
==ssDNA Qubit to quantify probe and template== | |||
==Probe calculations ppMALAT1== | ==Probe calculations ppMALAT1== |
Revision as of 20:15, 23 January 2014
Capturing ppMALAT1 template w ppMALT1 ordered form IDT
TBU GEL to verify probe template size
- Aim for 100ng/band
- 10bp Ladder
- ppMALAT1 (92nt, 100uM=3036ng/ul)
- MALAT1 Template (45nt, 100uM=1485ng/ul)
- ppillumina_155 (99nt, 100uM=3267ng/ul)
Sample | ng/ul | ul | ng/ul | ul | H2O |
ppMALAT1 | 3036 | 0.99 | 100 | 30 | 29.01 |
MALAT1 Template | 1485 | 1.35 | 100 | 20 | 18.65 |
ppillumina_155 | 3267 | 0.92 | 100 | 30 | 29.08 |
10BP | 1000 | 0.10 | 100 | 1 | 99 |