AlanFung:LabNotes/Capturing/2014-1-23: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Alan6017518
(Created page with "==Capturing ppMALAT1 template w ppMALT1 ordered form IDT== ==Probe calculations ppMALAT1==")
 
>Alan6017518
Line 1: Line 1:
==Capturing ppMALAT1 template w ppMALT1 ordered form IDT==
==Capturing ppMALAT1 template w ppMALT1 ordered form IDT==
==TBU GEL to verify probe template size==
*Aim for 100ng/band
*10bp Ladder
*ppMALAT1 (92nt, 100uM=3036ng/ul)
*MALAT1 Template (45nt, 100uM=1485ng/ul)
*ppillumina_155 (99nt, 100uM=3267ng/ul)
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
|-  align="center"
| width="274" height="13" | Sample
| width="75" | ng/ul
|style="background-color:#EBF1DE" width="75" | ul
| width="273" | ng/ul
| width="147" | ul
|style="background-color:#EBF1DE" width="75" | H2O
|-  align="center"
| height="13" | ppMALAT1
| align="center" | 3036
|style="background-color:#EBF1DE" align="center" | 0.99
| align="center" | 100
| align="center" | 30
|style="background-color:#EBF1DE" align="center" | 29.01
|-  align="center"
| height="13" | MALAT1 Template
| align="center" | 1485
|style="background-color:#EBF1DE" align="center" | 1.35
| align="center" | 100
| align="center" | 20
|style="background-color:#EBF1DE" align="center" | 18.65
|-  align="center"
| height="13" | ppillumina_155
| align="center" | 3267
|style="background-color:#EBF1DE" align="center" | 0.92
| align="center" | 100
| align="center" | 30
|style="background-color:#EBF1DE" align="center" | 29.08
|-  align="center"
| height="13" | 10BP
| align="center" | 1000
|style="background-color:#EBF1DE" align="center" | 0.10
| align="center" | 100
| align="center" | 1
|style="background-color:#EBF1DE" align="center" | 99
|}
==ssDNA Qubit to quantify probe and template==
==Probe calculations ppMALAT1==
==Probe calculations ppMALAT1==

Revision as of 20:15, 23 January 2014

Capturing ppMALAT1 template w ppMALT1 ordered form IDT

TBU GEL to verify probe template size

  • Aim for 100ng/band
  • 10bp Ladder
  • ppMALAT1 (92nt, 100uM=3036ng/ul)
  • MALAT1 Template (45nt, 100uM=1485ng/ul)
  • ppillumina_155 (99nt, 100uM=3267ng/ul)
Sample ng/ul ul ng/ul ul H2O
ppMALAT1 3036 0.99 100 30 29.01
MALAT1 Template 1485 1.35 100 20 18.65
ppillumina_155 3267 0.92 100 30 29.08
10BP 1000 0.10 100 1 99


ssDNA Qubit to quantify probe and template

Probe calculations ppMALAT1