Matt:LabNotes/2014-1-31: Difference between revisions
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>Mzcai |
>Mzcai |
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| align="center" style="background:#f0f0f0;"|'''% of Cy3''' | | align="center" style="background:#f0f0f0;"|'''% of Cy3''' | ||
|- | |- | ||
| C9||220nt||711||129|| | | C9||220nt||711||129||18.14 | ||
|- | |- | ||
| D9||300nt||357||203|| | | D9||300nt||357||203||56.86 | ||
|- | |- | ||
| E9||510nt||4449||842|| | | E9||510nt||4449||842||18.923 | ||
|- | |- | ||
| F9||1000nt||13295||14654|| | | F9||1000nt||13295||14654||110.22 | ||
|- | |- | ||
| ||||||Average % of Cy3:|| | | ||||||Average % of Cy3:||51.04 | ||
|} | |} | ||
Revision as of 22:09, 6 February 2014
Shifted FISSEQ vs FISSEQ_Adpt Signal Count in Same Wells
- Previously compared the two dyes in adjacent wells Matt:LabNotes/2014-1-28
- Here I strip FISSEQ_Adpt from before and add Shifted FISSEQ to column 9
- Put Shifted FISSEQ_2ndRCAprimerDye in columns 4 and 9
- Put RAB7A_1stRolonyFISH in columns 3 and 10
Well | RT primer | Cy3 | Shifted | % of Cy3 |
C9 | 220nt | 711 | 129 | 18.14 |
D9 | 300nt | 357 | 203 | 56.86 |
E9 | 510nt | 4449 | 842 | 18.923 |
F9 | 1000nt | 13295 | 14654 | 110.22 |
Average % of Cy3: | 51.04 |
RT_RAB7A Primers and the Number of RAB7A Primary Rolonies
- Using RAB7A_1stRolonyFISH (ATTO550)
C10_550: 24 D10_550: 24 E10_550: 13 F10_550: 35