Blue:RNA-Seq Libraries:2014: Difference between revisions

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{| {{table}} border=1
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| align="center" style="background:#f0f0f0;"|'''Library'''
| align="center" style="background:#f0f0f0;"|'''Library'''
| align="center" style="background:#f0f0f0;"|'''Experiment'''
| align="center" style="background:#f0f0f0;"|'''Condition'''
| align="center" style="background:#f0f0f0;"|'''N2.ID'''
| align="center" style="background:#f0f0f0;"|'''RNA Spike-in'''
| align="center" style="background:#f0f0f0;"|'''T20VN.ID'''
| align="center" style="background:#f0f0f0;"|'''T20-30 (Barcode)'''
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''TSO'''
| align="center" style="background:#f0f0f0;"|'''TSO'''
| align="center" style="background:#f0f0f0;"|'''Condition'''
| align="center" style="background:#f0f0f0;"|'''PCR Primers'''
| align="center" style="background:#f0f0f0;"|'''Seq Run'''
| align="center" style="background:#f0f0f0;"|'''Library Processing'''
| align="center" style="background:#f0f0f0;"|'''Lane'''
| align="center" style="background:#f0f0f0;"|'''N2/Nextera Index'''
| align="center" style="background:#f0f0f0;"|'''Data Storage Location'''
| align="center" style="background:#f0f0f0;"|'''Run Type, Minmum BP'''
| align="center" style="background:#f0f0f0;"|'''Analysis'''
| align="center" style="background:#f0f0f0;"|'''Read 1 Sequencing Primer'''
| align="center" style="background:#f0f0f0;"|'''Index Primer'''
| align="center" style="background:#f0f0f0;"|'''Read 2 Sequencing Primer'''
|-
| BL_totoRNAseq_1ng-100pg-Mg-UHRR_Mar05||1ng UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||1||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A
|-
| ||1ng UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||2||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A
|-
| ||100pg UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E6 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||3||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A
|-
| ||100pg UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E6 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||4||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A
|-
| ||NTC; 2min Mg Frag; Enz. PAP 5min||1:2E7 ERCC||T20VN_id11||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||6||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A
|-
| BL_totoRNAseq_1ng-100pg-RIII-UHRR_Mar05||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||7||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A
|-
| ||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||8||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A
|-
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||9||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A
|-
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||10||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A
|-
| ||NTC; 1min 0.1U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||T20VN_id11||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||12||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A
|-
| BL_totoRNAseq_10x10pg-Mg-UHRR_Mar05||10x10pg UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E7 ERCC||T20VN_id1-10||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||5||PE 50+7+8||STRT-SEQ v4||N2IndSeq||N2RevSeq2
|-
| BL_totoRNAseq_10x10pg-RIII-UHRR_Mar05||10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||T20VN_id1-10||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||11||PE 50+7+8||STRT-SEQ v4||N2IndSeq||N2RevSeq2
|-
| BL_totoSmartSeq2_1ng-100pg-10pg-RIII-UHRR_Mar05||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S501/N701||PE 50+7+8||HP10||HP12||HP11
|-
| ||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S502/N702||PE 50+7+8||HP10||HP12||HP11
|-
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S503/N703||PE 50+7+8||HP10||HP12||HP11
|-
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S504/N704||PE 50+7+8||HP10||HP12||HP11
|-
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S505/N705||PE 50+7+8||HP10||HP12||HP11
|-
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S506/N706||PE 50+7+8||HP10||HP12||HP11
|-
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S507/N707||PE 50+7+8||HP10||HP12||HP11
|-
| ||NTC; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S508/N708||PE 50+7+8||HP10||HP12||HP11
|-
|-
|BL_totoRNAseq_10pgUHRR/hNPC_Jan13||[[Blue:RNA-Seq_Experiments:01132014|01132014]]||15||1-10||10x10pg UHRR(ERCC)||R06||0.1U RnaseIII 15s/4min PAP||HL||Lanes ||||
| BL_totoQuartzSeq_1ng-100pg-10pg-RIII-UHRR_Mar05||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S501/N709||PE 50+7+8||HP10||HP12||HP11
|-
|-
|||[[Blue:RNA-Seq_Experiments:01132014|01132014]]||16||1-8,10-12||10xhNPC SC(ERCC)||R06||0.1U RnaseIII 15s/4min PAP||HL||Lanes ||||
| ||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S502/N710||PE 50+7+8||HP10||HP12||HP11
|-
|-
|||[[Blue:RNA-Seq_Experiments:01132014|01132014]]||17||13-23||10xhNPC SC(ERCC)||R06||0.1U RnaseIII 15s/4min PAP||HL||Lanes ||||
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N711||PE 50+7+8||HP10||HP12||HP11
|-
|-
|BL_totoRNAseq_10pgUHRR_Jan22||[[Blue:RNA-Seq_Experiments:01222014|01222014]]||81||1-10||10x10pg UHRR(ERCC)||R06||0.1U RnaseIII 1min/4min PAP/RT 37C 60min||HL||Lanes ||||
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S504/N712||PE 50+7+8||HP10||HP12||HP11
|-
|-
|||[[Blue:RNA-Seq_Experiments:01222014|01222014]]||82||1-10||10x10pg UHRR(ERCC)||R06||0.05U RnaseIII 1min/4min PAP/RT 37C 60min||HL||Lanes ||||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S505/N701||PE 50+7+8||HP10||HP12||HP11
|-
|-
|||[[Blue:RNA-Seq_Experiments:01222014|01222014]]||83||1-10||10x10pg UHRR(ERCC)||R06||0.01U RnaseIII 1min/4min PAP/RT 37C 60min||HL||Lanes ||||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S506/N702||PE 50+7+8||HP10||HP12||HP11
|-
|-
|||[[Blue:RNA-Seq_Experiments:01222014|01222014]]||84-87||1||1ng UHRR(ERCC)/RT 37C 60min||R06||0.1U RnaseIII 1min/10min PAP||HL||Lanes ||||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S507/N703||PE 50+7+8||HP10||HP12||HP11
|-
|-
|BL_totoRNAseq_10pgUHRR/hNPC_Jan29||[[Blue:RNA-Seq_Experiments:01292014|01292014]]||1||1-10||10x10pg UHRR(ERCC)||R06||0.05U RnaseIII 1min/4min PAP/RT 42C 30min||HL||Lanes ||||
| ||NTC; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S508/N704||PE 50+7+8||HP10||HP12||HP11
|-
|-
|||[[Blue:RNA-Seq_Experiments:01292014|01292014]]||2||1-10||10xhNPC SC(ERCC)||R06||0.05U RnaseIII 1min/4min PAP/RT 37C 60min||HL||Lanes ||||
| BL_totoRNAseq_10x10pg_0.01-0.1URIII_UHRR_Jan16||10x10pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||T20VN_id1-10||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||81||PE 50+7+8||STRT-SEQ v4||N2IndSeq||N2RevSeq2
|-
|-
|||[[Blue:RNA-Seq_Experiments:01292014|01292014]]||3||1-10||10xhNPC SC(ERCC)||R06||0.05U RnaseIII 1min/4min PAP/RT 37C 60min||HL||Lanes ||||
| ||10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||T20VN_id1-10||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||82||PE 50+7+8||STRT-SEQ v4||N2IndSeq||N2RevSeq2
|-
|-
|||[[Blue:RNA-Seq_Experiments:01292014|01292014]]||4-7||1-10||1ng UHRR(ERCC)||R06||0.1U RnaseIII 1min/10min PAP/RT 42C 30min||HL||Lanes ||||
| ||10x10pg UHRR; 1min 0.01U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||T20VN_id1-10||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||83||PE 50+7+8||STRT-SEQ v4||N2IndSeq||N2RevSeq2
|-
|-
|
|}
|}

Revision as of 20:17, 8 May 2014

Library Condition RNA Spike-in T20-30 (Barcode) TSO PCR Primers Library Processing N2/Nextera Index Run Type, Minmum BP Read 1 Sequencing Primer Index Primer Read 2 Sequencing Primer
BL_totoRNAseq_1ng-100pg-Mg-UHRR_Mar05 1ng UHRR; 2min Mg Frag; Enz. PAP 5min 1:2E5 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 1 SE 50+7 STRT-SEQ v3 N2IndSeq N/A
1ng UHRR; 2min Mg Frag; Enz. PAP 5min 1:2E5 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 2 SE 50+7 STRT-SEQ v3 N2IndSeq N/A
100pg UHRR; 2min Mg Frag; Enz. PAP 5min 1:2E6 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 3 SE 50+7 STRT-SEQ v3 N2IndSeq N/A
100pg UHRR; 2min Mg Frag; Enz. PAP 5min 1:2E6 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 4 SE 50+7 STRT-SEQ v3 N2IndSeq N/A
NTC; 2min Mg Frag; Enz. PAP 5min 1:2E7 ERCC T20VN_id11 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 6 SE 50+7 STRT-SEQ v3 N2IndSeq N/A
BL_totoRNAseq_1ng-100pg-RIII-UHRR_Mar05 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 7 SE 50+7 STRT-SEQ v3 N2IndSeq N/A
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 8 SE 50+7 STRT-SEQ v3 N2IndSeq N/A
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 9 SE 50+7 STRT-SEQ v3 N2IndSeq N/A
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC T20VN_id1 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 10 SE 50+7 STRT-SEQ v3 N2IndSeq N/A
NTC; 1min 0.1U RIII Frag; Affy. PAP 4min 1:2E7 ERCC T20VN_id11 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 12 SE 50+7 STRT-SEQ v3 N2IndSeq N/A
BL_totoRNAseq_10x10pg-Mg-UHRR_Mar05 10x10pg UHRR; 2min Mg Frag; Enz. PAP 5min 1:2E7 ERCC T20VN_id1-10 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 5 PE 50+7+8 STRT-SEQ v4 N2IndSeq N2RevSeq2
BL_totoRNAseq_10x10pg-RIII-UHRR_Mar05 10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC T20VN_id1-10 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 11 PE 50+7+8 STRT-SEQ v4 N2IndSeq N2RevSeq2
BL_totoSmartSeq2_1ng-100pg-10pg-RIII-UHRR_Mar05 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S501/N701 PE 50+7+8 HP10 HP12 HP11
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S502/N702 PE 50+7+8 HP10 HP12 HP11
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S503/N703 PE 50+7+8 HP10 HP12 HP11
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S504/N704 PE 50+7+8 HP10 HP12 HP11
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S505/N705 PE 50+7+8 HP10 HP12 HP11
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S506/N706 PE 50+7+8 HP10 HP12 HP11
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S507/N707 PE 50+7+8 HP10 HP12 HP11
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Smart2_T30VN Smart2_TSO IS Nextera XT S508/N708 PE 50+7+8 HP10 HP12 HP11
BL_totoQuartzSeq_1ng-100pg-10pg-RIII-UHRR_Mar05 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S501/N709 PE 50+7+8 HP10 HP12 HP11
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E5 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S502/N710 PE 50+7+8 HP10 HP12 HP11
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S503/N711 PE 50+7+8 HP10 HP12 HP11
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min 1:2E6 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S504/N712 PE 50+7+8 HP10 HP12 HP11
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S505/N701 PE 50+7+8 HP10 HP12 HP11
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S506/N702 PE 50+7+8 HP10 HP12 HP11
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S507/N703 PE 50+7+8 HP10 HP12 HP11
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC Quartz-RT (WTA) Quartz_Tagging Quartz Suppression Nextera XT S508/N704 PE 50+7+8 HP10 HP12 HP11
BL_totoRNAseq_10x10pg_0.01-0.1URIII_UHRR_Jan16 10x10pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 4min 1:2E7 ERCC T20VN_id1-10 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 81 PE 50+7+8 STRT-SEQ v4 N2IndSeq N2RevSeq2
10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min 1:2E7 ERCC T20VN_id1-10 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 82 PE 50+7+8 STRT-SEQ v4 N2IndSeq N2RevSeq2
10x10pg UHRR; 1min 0.01U RIII Frag; Affy. PAP 4min 1:2E7 ERCC T20VN_id1-10 TSO.r06 P1-STRT/PCR_R_N2_idx n/a 83 PE 50+7+8 STRT-SEQ v4 N2IndSeq N2RevSeq2