Blue:RNA-Seq Libraries:2014: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''Read 2 Sequencing Primer''' | | align="center" style="background:#f0f0f0;"|'''Read 2 Sequencing Primer''' | ||
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| BL_totoRNAseq_1ng-100pg-Mg-UHRR_Mar05||1ng UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||1||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A | | [http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:03052014#totoRNASeq_Library_Preparation BL_totoRNAseq_1ng-100pg-Mg-UHRR_Mar05]||1ng UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||1||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A | ||
|- | |- | ||
| ||1ng UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||2||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A | | ||1ng UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||2||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A |
Revision as of 21:47, 12 May 2014
Library | Condition | RNA Spike-in | T20-30 (Barcode) | TSO | PCR Primers | Library Processing | N2/Nextera Index | Run Type, Minmum BP | Read 1 Sequencing Primer | Index Primer | Read 2 Sequencing Primer |
BL_totoRNAseq_1ng-100pg-Mg-UHRR_Mar05 | 1ng UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 1 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A |
1ng UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 2 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | |
100pg UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 3 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | |
100pg UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 4 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | |
NTC; 2min Mg Frag; Enz. PAP 5min | 1:2E7 ERCC | T20VN_id11 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 6 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | |
BL_totoRNAseq_1ng-100pg-RIII-UHRR_Mar05 | 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 7 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A |
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 8 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 9 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 10 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | |
NTC; 1min 0.1U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id11 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 12 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | |
BL_totoRNAseq_10x10pg-Mg-UHRR_Mar05 | 10x10pg UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 5 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 |
BL_totoRNAseq_10x10pg-RIII-UHRR_Mar05 | 10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 11 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 |
BL_totoSmartSeq2_1ng-100pg-10pg-RIII-UHRR_Mar05 | 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S501/N701 | PE 50+7+8 | HP10 | HP12 | HP11 |
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S502/N702 | PE 50+7+8 | HP10 | HP12 | HP11 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S503/N703 | PE 50+7+8 | HP10 | HP12 | HP11 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S504/N704 | PE 50+7+8 | HP10 | HP12 | HP11 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S505/N705 | PE 50+7+8 | HP10 | HP12 | HP11 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S506/N706 | PE 50+7+8 | HP10 | HP12 | HP11 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S507/N707 | PE 50+7+8 | HP10 | HP12 | HP11 | |
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S508/N708 | PE 50+7+8 | HP10 | HP12 | HP11 | |
BL_totoQuartzSeq_1ng-100pg-10pg-RIII-UHRR_Mar05 | 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S501/N709 | PE 50+7+8 | HP10 | HP12 | HP11 |
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S502/N710 | PE 50+7+8 | HP10 | HP12 | HP11 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N711 | PE 50+7+8 | HP10 | HP12 | HP11 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S504/N712 | PE 50+7+8 | HP10 | HP12 | HP11 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S505/N701 | PE 50+7+8 | HP10 | HP12 | HP11 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S506/N702 | PE 50+7+8 | HP10 | HP12 | HP11 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S507/N703 | PE 50+7+8 | HP10 | HP12 | HP11 | |
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S508/N704 | PE 50+7+8 | HP10 | HP12 | HP11 | |
BL_totoRNAseq_10x10pg_0.01-0.1URIII_UHRR_Jan22 | 10x10pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 81 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 |
10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 82 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | |
10x10pg UHRR; 1min 0.01U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 83 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | |