Blue:RNA-Seq Libraries:2014: Difference between revisions
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Library''' | | align="center" style="background:#f0f0f0;"|'''Library''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Condition''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''RNA Spike-in''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''T20-30 (Barcode)''' | ||
| align="center" style="background:#f0f0f0;"|'''TSO''' | | align="center" style="background:#f0f0f0;"|'''TSO''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''PCR Primers''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Library Processing''' | ||
| align="center" style="background:#f0f0f0;"|'''Lane''' | | align="center" style="background:#f0f0f0;"|'''N2/Nextera Index''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Run Type, Minmum BP''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Read 1 Sequencing Primer''' | ||
| align="center" style="background:#f0f0f0;"|'''Index Primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Read 2 Sequencing Primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Seq Lane''' | |||
| align="center" style="background:#f0f0f0;"|'''File''' | |||
| align="center" style="background:#f0f0f0;"|'''File Location''' | |||
|- | |||
| [http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:03052014 BL_totoRNAseq_1ng-100pg-Mg-UHRR_Mar05]||1ng UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||1||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A||Lane 5||s_5_1_Indx01.txt||140514_HL166 | |||
|- | |||
| ||1ng UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||2||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A||Lane 5||s_5_1_Indx02.txt||140514_HL166 | |||
|- | |||
| ||100pg UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E6 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||3||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A||Lane 5||s_5_1_Indx03.txt||140514_HL166 | |||
|- | |||
| ||100pg UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E6 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||4||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A||Lane 5||s_5_1_Indx04.txt||140514_HL166 | |||
|- | |||
| ||NTC; 2min Mg Frag; Enz. PAP 5min||1:2E7 ERCC||T20VN_id11||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||6||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A||Lane 5||s_5_1_Indx06.txt||140514_HL166 | |||
|- | |||
| [http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:03052014 BL_totoRNAseq_1ng-100pg-RIII-UHRR_Mar05]||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||7||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A||Lane 5||s_5_1_Indx07.txt||140514_HL166 | |||
|- | |||
| ||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||8||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A||Lane 5||s_5_1_Indx08.txt||140514_HL166 | |||
|- | |||
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||9||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A||Lane 5||s_5_1_Indx09.txt||140514_HL166 | |||
|- | |||
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||T20VN_id1||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||10||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A||Lane 5||s_5_1_Indx10.txt||140514_HL166 | |||
|- | |||
| ||NTC; 1min 0.1U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||T20VN_id11||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||12||SE 50+7||STRT-SEQ v3||N2IndSeq||N/A||Lane 5||s_5_1_Indx12.txt||140514_HL166 | |||
|- | |||
| [http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:03052014 BL_totoRNAseq_10x10pg-Mg-UHRR_Mar05]||10x10pg UHRR; 2min Mg Frag; Enz. PAP 5min||1:2E7 ERCC||T20VN_id1-10||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||5||PE 50+7+8||STRT-SEQ v4||N2IndSeq||N2RevSeq2||Lane 5||s_5_1_Indx05.txt||140514_HL166 | |||
|- | |||
| [http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:03052014 BL_totoRNAseq_10x10pg-RIII-UHRR_Mar05]||10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||T20VN_id1-10||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||11||PE 50+7+8||STRT-SEQ v4||N2IndSeq||N2RevSeq2||Lane 1||s_1_1_Indx11.txt||140514_HL166 | |||
|- | |||
| [http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:03052014 BL_totoSmartSeq2_1ng-100pg-10pg-RIII-UHRR_Mar05]||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S501/N701||PE 50+7+8||HP10||HP12||HP11||Lane 6||s_6_1_N701.txt||140514_HL166 | |||
|- | |||
| ||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S502/N702||PE 50+7+8||HP10||HP12||HP11||Lane 6||s_6_1_N702.txt||140514_HL166 | |||
|- | |||
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S503/N703||PE 50+7+8||HP10||HP12||HP11||Lane 6||s_6_1_N703.txt||140514_HL166 | |||
|- | |||
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S504/N704||PE 50+7+8||HP10||HP12||HP11||Lane 6||s_6_1_N704.txt||140514_HL166 | |||
|- | |||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S505/N705||PE 50+7+8||HP10||HP12||HP11||Lane 6||s_6_1_N705.txt||140514_HL166 | |||
|- | |||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S506/N706||PE 50+7+8||HP10||HP12||HP11||Lane 6||s_6_1_N706.txt||140514_HL166 | |||
|- | |||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S507/N707||PE 50+7+8||HP10||HP12||HP11||Lane 6||s_6_1_N707.txt||140514_HL166 | |||
|- | |||
| ||NTC; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Smart2_T30VN||Smart2_TSO||IS||Nextera XT||S508/N708||PE 50+7+8||HP10||HP12||HP11||Lane 6||s_6_1_N708.txt||140514_HL166 | |||
|- | |||
| [http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:03052014 BL_totoQuartzSeq_1ng-100pg-10pg-RIII-UHRR_Mar05]||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S501/N709||PE 50+7+8||HP10||HP12||HP11||Lane 7||s_6_1_N709.txt||140514_HL166 | |||
|- | |||
| ||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S502/N710||PE 50+7+8||HP10||HP12||HP11||Lane 7||s_6_1_N710.txt||140514_HL166 | |||
|- | |||
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N711||PE 50+7+8||HP10||HP12||HP11||Lane 7||s_6_1_N711.txt||140514_HL166 | |||
|- | |||
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S504/N712||PE 50+7+8||HP10||HP12||HP11||Lane 7||s_6_1_N712.txt||140514_HL166 | |||
|- | |||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S505/N701||PE 50+7+8||HP10||HP12||HP11||Lane 7||s_6_1_N701.txt||140514_HL166 | |||
|- | |||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S506/N702||PE 50+7+8||HP10||HP12||HP11||Lane 7||s_6_1_N702.txt||140514_HL166 | |||
|- | |||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S507/N703||PE 50+7+8||HP10||HP12||HP11||Lane 7||s_6_1_N703.txt||140514_HL166 | |||
|- | |||
| ||NTC; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S508/N704||PE 50+7+8||HP10||HP12||HP11||Lane 7||s_6_1_N704.txt||140514_HL166 | |||
|- | |||
| [http://genome-tech.ucsd.edu/LabNotes/index.php/Blue:RNA-Seq_Experiments:01222014 BL_totoRNAseq_10x10pg_0.01-0.1URIII_UHRR_Jan22]||10x10pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||T20VN_id1-10||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||81||PE 50+7+8||STRT-SEQ v4||N2IndSeq||N2RevSeq2||Lane 2, 8||s_2(8)_1_Indx81.txt||140514_HL166 | |||
|- | |||
| ||10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||T20VN_id1-10||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||82||PE 50+7+8||STRT-SEQ v4||N2IndSeq||N2RevSeq2||Lane 2, 8||s_2(8)_1_Indx82.txt||140514_HL166 | |||
|- | |||
| ||10x10pg UHRR; 1min 0.01U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||T20VN_id1-10||TSO.r06||P1-STRT/PCR_R_N2_idx||n/a||83||PE 50+7+8||STRT-SEQ v4||N2IndSeq||N2RevSeq2||Lane 2, 8||s_2(8)_1_Indx83.txt||140514_HL166 | |||
|- | |||
| BL-totoSTRTseq-1ng_100pg_10pg_RIII_UHRR-Mar05||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||C1-P1-T31||C1-P1-RNA-TSO||C1-P1-PCR-2||Sten Tagmentation||C1-TN5-01||SE 50+7||C1-P1-PCR-2||C1-TN5-U||N/A||Lane 1,2||s_1_1_StenTn5-id01.txt||/home/kunzhang/seqStore/140701_HL169 | |||
|- | |||
| ||1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E5 ERCC||C1-P1-T31||C1-P1-RNA-TSO||C1-P1-PCR-2||Sten Tagmentation||C1-TN5-02||SE 50+7||C1-P1-PCR-2||C1-TN5-U||N/A||Lane 1,2||s_1_1_StenTn5-id02.txt||/home/kunzhang/seqStore/140701_HL169 | |||
|- | |||
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||C1-P1-T31||C1-P1-RNA-TSO||C1-P1-PCR-2||Sten Tagmentation||C1-TN5-03||SE 50+7||C1-P1-PCR-2||C1-TN5-U||N/A||Lane 1,2||s_1_1_StenTn5-id03.txt||/home/kunzhang/seqStore/140701_HL169 | |||
|- | |||
| ||100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min||1:2E6 ERCC||C1-P1-T31||C1-P1-RNA-TSO||C1-P1-PCR-2||Sten Tagmentation||C1-TN5-04||SE 50+7||C1-P1-PCR-2||C1-TN5-U||N/A||Lane 1,2||s_1_1_StenTn5-id04.txt||/home/kunzhang/seqStore/140701_HL169 | |||
|- | |||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||C1-P1-T31||C1-P1-RNA-TSO||C1-P1-PCR-2||Sten Tagmentation||C1-TN5-05||SE 50+7||C1-P1-PCR-2||C1-TN5-U||N/A||Lane 1,2||s_1_1_StenTn5-id05.txt||/home/kunzhang/seqStore/140701_HL169 | |||
|- | |||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||C1-P1-T31||C1-P1-RNA-TSO||C1-P1-PCR-2||Sten Tagmentation||C1-TN5-06||SE 50+7||C1-P1-PCR-2||C1-TN5-U||N/A||Lane 1,2||s_1_1_StenTn5-id06.txt||/home/kunzhang/seqStore/140701_HL169 | |||
|- | |||
| ||10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||C1-P1-T31||C1-P1-RNA-TSO||C1-P1-PCR-2||Sten Tagmentation||C1-TN5-07||SE 50+7||C1-P1-PCR-2||C1-TN5-U||N/A||Lane 1,2||s_1_1_StenTn5-id07.txt||/home/kunzhang/seqStore/140701_HL169 | |||
|- | |||
| ||NTC; 1min 0.05U RIII Frag; Affy. PAP 4min||1:2E7 ERCC||C1-P1-T31||C1-P1-RNA-TSO||C1-P1-PCR-2||Sten Tagmentation||C1-TN5-08||SE 50+7||C1-P1-PCR-2||C1-TN5-U||N/A||Lane 1,2||s_1_1_StenTn5-id08.txt||/home/kunzhang/seqStore/140701_HL169 | |||
|- | |||
| BL-totoQuartzSeq-1ng_11_15cycles_UHRR-Jun06||1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 11cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N701||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N701.txt||/home/kunzhang/seqStore/140701_HL169 | |||
|- | |- | ||
| | | ||1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 11cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N702||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N702.txt||/home/kunzhang/seqStore/140701_HL169 | ||
|- | |- | ||
||| | | ||1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 13cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N703||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N703.txt||/home/kunzhang/seqStore/140701_HL169 | ||
|- | |- | ||
||| | | ||1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 13cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N704||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N704.txt||/home/kunzhang/seqStore/140701_HL169 | ||
|- | |- | ||
| | | ||1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 15cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N705||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N705.txt||/home/kunzhang/seqStore/140701_HL169 | ||
|- | |- | ||
||| | | ||1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 15cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N706||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N706.txt||/home/kunzhang/seqStore/140701_HL169 | ||
|- | |- | ||
||| | | ||NTC; 1min 0.1U RIII Frag; Affy.PAP, 15cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N707||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N707.txt||/home/kunzhang/seqStore/140701_HL169 | ||
|- | |- | ||
||| | | ||NTC; 1min 0.1U RIII Frag; Affy.PAP, 15cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N708||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N708.txt||/home/kunzhang/seqStore/140701_HL169 | ||
|- | |- | ||
| | | ||1ng UHRR; Mg Frag; Affy.PAP, 15cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N709||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N709.txt||/home/kunzhang/seqStore/140701_HL169 | ||
|- | |- | ||
||| | | ||1ng UHRR; Mg Frag; Affy.PAP, 15cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N710||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N7010.txt||/home/kunzhang/seqStore/140701_HL169 | ||
|- | |- | ||
||| | | ||NTC; Mg Frag; Affy.PAP, 15cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N711||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N7011.txt||/home/kunzhang/seqStore/140701_HL169 | ||
|- | |- | ||
||| | | ||NTC; Mg Frag; Affy.PAP, 15cycles||1:2E5 ERCC||Quartz-RT (WTA)||Quartz_Tagging||Quartz Suppression||Nextera XT||S503/N712||SE 50+7||NeteraXT_R1||NeteraXT_i7Ind||n/a||Lane 6||s_6_1_N7012.txt||/home/kunzhang/seqStore/140701_HL169 | ||
|- | |- | ||
|} | |} |
Latest revision as of 20:12, 7 July 2014
Library | Condition | RNA Spike-in | T20-30 (Barcode) | TSO | PCR Primers | Library Processing | N2/Nextera Index | Run Type, Minmum BP | Read 1 Sequencing Primer | Index Primer | Read 2 Sequencing Primer | Seq Lane | File | File Location |
BL_totoRNAseq_1ng-100pg-Mg-UHRR_Mar05 | 1ng UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 1 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx01.txt | 140514_HL166 |
1ng UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 2 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx02.txt | 140514_HL166 | |
100pg UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 3 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx03.txt | 140514_HL166 | |
100pg UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 4 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx04.txt | 140514_HL166 | |
NTC; 2min Mg Frag; Enz. PAP 5min | 1:2E7 ERCC | T20VN_id11 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 6 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx06.txt | 140514_HL166 | |
BL_totoRNAseq_1ng-100pg-RIII-UHRR_Mar05 | 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 7 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx07.txt | 140514_HL166 |
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 8 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx08.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 9 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx09.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | T20VN_id1 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 10 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx10.txt | 140514_HL166 | |
NTC; 1min 0.1U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id11 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 12 | SE 50+7 | STRT-SEQ v3 | N2IndSeq | N/A | Lane 5 | s_5_1_Indx12.txt | 140514_HL166 | |
BL_totoRNAseq_10x10pg-Mg-UHRR_Mar05 | 10x10pg UHRR; 2min Mg Frag; Enz. PAP 5min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 5 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | Lane 5 | s_5_1_Indx05.txt | 140514_HL166 |
BL_totoRNAseq_10x10pg-RIII-UHRR_Mar05 | 10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 11 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | Lane 1 | s_1_1_Indx11.txt | 140514_HL166 |
BL_totoSmartSeq2_1ng-100pg-10pg-RIII-UHRR_Mar05 | 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S501/N701 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N701.txt | 140514_HL166 |
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S502/N702 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N702.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S503/N703 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N703.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S504/N704 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N704.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S505/N705 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N705.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S506/N706 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N706.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S507/N707 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N707.txt | 140514_HL166 | |
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Smart2_T30VN | Smart2_TSO | IS | Nextera XT | S508/N708 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 6 | s_6_1_N708.txt | 140514_HL166 | |
BL_totoQuartzSeq_1ng-100pg-10pg-RIII-UHRR_Mar05 | 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S501/N709 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N709.txt | 140514_HL166 |
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S502/N710 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N710.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N711 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N711.txt | 140514_HL166 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S504/N712 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N712.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S505/N701 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N701.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S506/N702 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N702.txt | 140514_HL166 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S507/N703 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N703.txt | 140514_HL166 | |
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S508/N704 | PE 50+7+8 | HP10 | HP12 | HP11 | Lane 7 | s_6_1_N704.txt | 140514_HL166 | |
BL_totoRNAseq_10x10pg_0.01-0.1URIII_UHRR_Jan22 | 10x10pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 81 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | Lane 2, 8 | s_2(8)_1_Indx81.txt | 140514_HL166 |
10x10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 82 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | Lane 2, 8 | s_2(8)_1_Indx82.txt | 140514_HL166 | |
10x10pg UHRR; 1min 0.01U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | T20VN_id1-10 | TSO.r06 | P1-STRT/PCR_R_N2_idx | n/a | 83 | PE 50+7+8 | STRT-SEQ v4 | N2IndSeq | N2RevSeq2 | Lane 2, 8 | s_2(8)_1_Indx83.txt | 140514_HL166 | |
BL-totoSTRTseq-1ng_100pg_10pg_RIII_UHRR-Mar05 | 1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-01 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id01.txt | /home/kunzhang/seqStore/140701_HL169 |
1ng UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E5 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-02 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id02.txt | /home/kunzhang/seqStore/140701_HL169 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-03 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id03.txt | /home/kunzhang/seqStore/140701_HL169 | |
100pg UHRR; 1min 0.1U RIII Frag; Affy. PAP 10min | 1:2E6 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-04 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id04.txt | /home/kunzhang/seqStore/140701_HL169 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-05 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id05.txt | /home/kunzhang/seqStore/140701_HL169 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-06 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id06.txt | /home/kunzhang/seqStore/140701_HL169 | |
10pg UHRR; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-07 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id07.txt | /home/kunzhang/seqStore/140701_HL169 | |
NTC; 1min 0.05U RIII Frag; Affy. PAP 4min | 1:2E7 ERCC | C1-P1-T31 | C1-P1-RNA-TSO | C1-P1-PCR-2 | Sten Tagmentation | C1-TN5-08 | SE 50+7 | C1-P1-PCR-2 | C1-TN5-U | N/A | Lane 1,2 | s_1_1_StenTn5-id08.txt | /home/kunzhang/seqStore/140701_HL169 | |
BL-totoQuartzSeq-1ng_11_15cycles_UHRR-Jun06 | 1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 11cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N701 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N701.txt | /home/kunzhang/seqStore/140701_HL169 |
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 11cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N702 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N702.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 13cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N703 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N703.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 13cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N704 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N704.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N705 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N705.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; 1min 0.1U RIII Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N706 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N706.txt | /home/kunzhang/seqStore/140701_HL169 | |
NTC; 1min 0.1U RIII Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N707 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N707.txt | /home/kunzhang/seqStore/140701_HL169 | |
NTC; 1min 0.1U RIII Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N708 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N708.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; Mg Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N709 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N709.txt | /home/kunzhang/seqStore/140701_HL169 | |
1ng UHRR; Mg Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N710 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N7010.txt | /home/kunzhang/seqStore/140701_HL169 | |
NTC; Mg Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N711 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N7011.txt | /home/kunzhang/seqStore/140701_HL169 | |
NTC; Mg Frag; Affy.PAP, 15cycles | 1:2E5 ERCC | Quartz-RT (WTA) | Quartz_Tagging | Quartz Suppression | Nextera XT | S503/N712 | SE 50+7 | NeteraXT_R1 | NeteraXT_i7Ind | n/a | Lane 6 | s_6_1_N7012.txt | /home/kunzhang/seqStore/140701_HL169 |