Daniel:Notebook/HiResChrPaint/2014-2-13: Difference between revisions
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>Djacobse (Created page with "=Multi-Color FISH (Started 02/08/2014)= Back to Calendar ==Cot1 Labeling ([[Daniel:Notebook/HiRe...") |
>Djacobse No edit summary |
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==Cot1 Labeling ([[Daniel:Notebook/HiResChrPaint/2014-2-12|Started 02/12/2014]])== | ==Cot1 Labeling ([[Daniel:Notebook/HiResChrPaint/2014-2-12|Started 02/12/2014]])== | ||
#Remove samples from -80C and centrifuge for 20 minutes at 12000 RPM at 4 C | |||
#Remove supernatant and replace with 750uL 75% EtOH | |||
#Centrifuge for 10 minutes at 12000 RPM at 4 C | |||
#Remove supernatant and leave in hood to dry | |||
#Resuspend each sample in 20 uL nfH2O | |||
##Measure concentration in nanodrop | |||
##Run on TBE gel to ensure correct size distribution | |||
#Pool all high MW samples and all low MW samples | |||
===Results=== | |||
'''Nanodrop Results''' | |||
'''Gel Image''' |
Revision as of 19:26, 13 February 2014
Multi-Color FISH (Started 02/08/2014)
Cot1 Labeling (Started 02/12/2014)
- Remove samples from -80C and centrifuge for 20 minutes at 12000 RPM at 4 C
- Remove supernatant and replace with 750uL 75% EtOH
- Centrifuge for 10 minutes at 12000 RPM at 4 C
- Remove supernatant and leave in hood to dry
- Resuspend each sample in 20 uL nfH2O
- Measure concentration in nanodrop
- Run on TBE gel to ensure correct size distribution
- Pool all high MW samples and all low MW samples
Results
Nanodrop Results
Gel Image