Daniel:Notebook/HiResChrPaint/2014-2-13: Difference between revisions
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#Resuspend each sample in 20 uL nfH2O | #Resuspend each sample in 20 uL nfH2O | ||
##Measure concentration in nanodrop | ##Measure concentration in nanodrop | ||
##Run on TBE gel to ensure correct size distribution | ##Run on [[Daniel:Protocols/TBEgel|TBE gel]] to ensure correct size distribution | ||
#Pool all high MW samples and all low MW samples | #Pool all high MW samples and all low MW samples | ||
Revision as of 19:27, 13 February 2014
Multi-Color FISH (Started 02/08/2014)
Cot1 Labeling (Started 02/12/2014)
- Remove samples from -80C and centrifuge for 20 minutes at 12000 RPM at 4 C
- Remove supernatant and replace with 750uL 75% EtOH
- Centrifuge for 10 minutes at 12000 RPM at 4 C
- Remove supernatant and leave in hood to dry
- Resuspend each sample in 20 uL nfH2O
- Measure concentration in nanodrop
- Run on TBE gel to ensure correct size distribution
- Pool all high MW samples and all low MW samples
Results
Nanodrop Results
Gel Image