Daniel:Notebook/HiResChrPaint/2014-2-9: Difference between revisions
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=Multi-Color FISH ([[Daniel:Notebook/HiResChrPaint/2014-2-8|Started 02/08/2014]])= | |||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | ||
==Cot1 Labeling ([[Daniel:Notebook/HiResChrPaint/2014-2-8|Started 02/08/2014]])== | |||
#Remove samples from -80C and centrifuge for 20 minutes at 12000 RPM at 4 C | |||
#Remove supernatant and replace with 750uL 75% EtOH | |||
#Centrifuge for 10 minutes at 12000 RPM at 4 C | |||
#Remove supernatant and leave in hood to dry | |||
#Resuspend each sample in 20 uL nfH2O | |||
##Measure concentration in nanodrop | |||
##Run on [[Daniel:Protocols/TBEgel|TBE gel]] to ensure correct size distribution | |||
#Pool all high MW samples and all low MW samples | |||
===Results=== | |||
'''Nanodrop Results''' | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt;font-weight:bold" align="center" | |||
| align="center" width="65" height="15" | | |||
| width="65" | High MW | |||
| width="65" | Low MW | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ng/uL | |||
| align="center" | 7.3 | |||
| align="center" | 8 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ug in 50 uL | |||
| align="center" | 0.4 | |||
| align="center" | 0.4 | |||
|} | |||
'''Gel Image''' | '''Gel Image''' | ||
[[Image:2014-02-09-Cot1_PostCut.jpg|500px]] | [[Image:2014-02-09-Cot1_PostCut.jpg|500px]] | ||
[[Category:HRCP]] [[Category:20140208]] |
Latest revision as of 18:20, 2 July 2014
Multi-Color FISH (Started 02/08/2014)[edit]
Cot1 Labeling (Started 02/08/2014)[edit]
- Remove samples from -80C and centrifuge for 20 minutes at 12000 RPM at 4 C
- Remove supernatant and replace with 750uL 75% EtOH
- Centrifuge for 10 minutes at 12000 RPM at 4 C
- Remove supernatant and leave in hood to dry
- Resuspend each sample in 20 uL nfH2O
- Measure concentration in nanodrop
- Run on TBE gel to ensure correct size distribution
- Pool all high MW samples and all low MW samples
Results[edit]
Nanodrop Results
High MW | Low MW | |
ng/uL | 7.3 | 8 |
ug in 50 uL | 0.4 | 0.4 |
Gel Image