EricChu:LabNotesMDA/2014-2-5: Difference between revisions
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>Ericchu (Created page with "==qPCR Alu and Mito of MDA generated libraries on 1-30-2014== * These libraries were prepared by tagmentation. * 1ul of each library was used in a 20ul qPCR reaction.") |
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* These libraries were prepared by tagmentation. | * These libraries were prepared by tagmentation. | ||
* 1ul of each library was used in a 20ul qPCR reaction. | * 1ul of each library was used in a 20ul qPCR reaction. | ||
* 1ul 10ng human gDNA was used as control. | |||
[[File:02052014qPCR.jpg|600px]] | |||
* Alu primers were labeled black and Mito primers were labeled red. | |||
* Alu curves of both libraries on 1-30-2014 (labeled Hi and Lo) were amplified later than their corresponding Mito curves. However, as seen in gDNA, Alu should be amplified earlier than Mito. | |||
* In this experiment, most of the DNA were coming from mitochondrial DNA rather than chromosomal DNA. |
Latest revision as of 02:39, 14 February 2014
qPCR Alu and Mito of MDA generated libraries on 1-30-2014[edit]
- These libraries were prepared by tagmentation.
- 1ul of each library was used in a 20ul qPCR reaction.
- 1ul 10ng human gDNA was used as control.
- Alu primers were labeled black and Mito primers were labeled red.
- Alu curves of both libraries on 1-30-2014 (labeled Hi and Lo) were amplified later than their corresponding Mito curves. However, as seen in gDNA, Alu should be amplified earlier than Mito.
- In this experiment, most of the DNA were coming from mitochondrial DNA rather than chromosomal DNA.