Daniel:Notebook/HiResChrPaint/2014-2-21: Difference between revisions
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#Aliquot 100 uL into each of | #Aliquot 100 uL into each of 6 qPCR tubes | ||
#PCR protocol: | #PCR protocol: | ||
##PCR 3 min 95C | ##PCR 3 min 95C | ||
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##72C 2 min | ##72C 2 min | ||
##16C hold | ##16C hold | ||
#Pooled 3 samples together each (300 uL each, | #Pooled 3 samples together each (300 uL each, 2 total) | ||
#Column Purification, elute with 40 uL EB | #Column Purification, elute with 40 uL EB | ||
#Measure concentrations in nanodrop | #Measure concentrations in nanodrop |
Revision as of 18:46, 21 February 2014
Multi-Color FISH (Started 02/08/2014)
qPCR of BSPP
Sample:
- BSPP 1-DMR 220K Exp 1 (10nM); 2/8/11 from Noi
- Reaction table
10 nM OligoPool | 2X Kapa SYBR qPCR MM | 100 uM AP1V61U | 100 uM AP2V6 | 2 mM aa-dUTP | H20 | Total Volume | |
per rxn | 0.4 | 50 | 0.4 | 0.4 | 2.5 | 46.3 | 100 |
BSPP 1 (6.1x) | 2.44 | 305 | 2.44 | 2.44 | 15.25 | 282.43 | 610 |
- Aliquot 100 uL into each of 6 qPCR tubes
- PCR protocol:
- PCR 3 min 95C
- 95C 3 sec
- 55C 30 sec
- 72C 20 sec
- plate read
- goto ii x17
- 72C 2 min
- 16C hold
- Pooled 3 samples together each (300 uL each, 2 total)
- Column Purification, elute with 40 uL EB
- Measure concentrations in nanodrop
qPCR Results
qPCR Curve
[[Image:|Image:]] Nanodrop Results