Daniel:Notebook/HiResChrPaint/2014-2-21: Difference between revisions
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'''Nanodrop Results''' | '''Nanodrop Results''' | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt;font-weight:bold" align="center" | |||
| width="65" height="15" | Sample | |||
| width="65" | ng/uL | |||
| width="65" | ug in 40 uL | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | BSPP1-A | |||
| align="center" | 73.9 | |||
| align="center" | 3.0 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | BSPP1-B | |||
| align="center" | 68.4 | |||
| align="center" | 2.7 | |||
|} |
Revision as of 23:21, 21 February 2014
Multi-Color FISH (Started 02/08/2014)
qPCR of BSPP
Sample:
- BSPP 1-DMR 220K Exp 1 (10nM); 2/8/11 from Noi
- Reaction table
10 nM OligoPool | 2X Kapa SYBR qPCR MM | 100 uM AP1V61U | 100 uM AP2V6 | 2 mM aa-dUTP | H20 | Total Volume | |
per rxn | 0.4 | 50 | 0.4 | 0.4 | 2.5 | 46.3 | 100 |
BSPP 1 (6.1x) | 2.44 | 305 | 2.44 | 2.44 | 15.25 | 282.43 | 610 |
- Aliquot 100 uL into each of 6 qPCR tubes
- PCR protocol:
- PCR 3 min 95C
- 95C 3 sec
- 55C 30 sec
- 72C 20 sec
- plate read
- goto ii x17
- 72C 2 min
- 16C hold
- Pooled 3 samples together each (300 uL each, 2 total)
- Column Purification, elute with 40 uL EB
- Measure concentrations in nanodrop
qPCR Results
qPCR Curve
Nanodrop Results
Sample | ng/uL | ug in 40 uL |
BSPP1-A | 73.9 | 3.0 |
BSPP1-B | 68.4 | 2.7 |