Song Chen:Daily/NOTES/2014-2-22: Difference between revisions
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===Cell density and DE differentiation=== | ===Cell density and DE differentiation=== | ||
Setting: Expose 1000 ms; PFBH 406, 10x obj | Setting: Expose 1000 ms; PFBH 406, 10x obj, | ||
<font color=red>SOX17</font><b>/</b><font color=blue>DAPI</font> | <font color=red>SOX17</font><b>/</b><font color=blue>DAPI</font> | ||
{|{{table}} | {|{{table}} |
Revision as of 02:37, 25 February 2014
Cell density and DE differentiation
Setting: Expose 1000 ms; PFBH 406, 10x obj, SOX17/DAPI
1x seeding density | 3x seeding density | 10x seeding density |
File:022214 H9DE 1x SOX17.png | File:022214 H9DE 3x SOX17.png | File:022214 H9DE 10x SOX17.png |
File:022214 H9DE 1x DAPI.png | File:022214 H9DE 3x DAPI.png | File:022214 H9DE 10x DAPI.png |
DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS
1x seeding density: D6-D7 hES passage at 1:12-1:15 ratio
High seeding density results in high efficient DE differentiation