Song Chen:Daily/NOTES/2014-2-22: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Chensong
No edit summary
>Chensong
No edit summary
Line 1: Line 1:
===Cell density and DE differentiation===
===Cell density and DE differentiation===
Setting: Expose 1000 ms; PFBH 406, 10x obj
Setting: Expose 1000 ms; PFBH 406, 10x obj,
<font color=red>SOX17</font><b>/</b><font color=blue>DAPI</font>
<font color=red>SOX17</font><b>/</b><font color=blue>DAPI</font>
{|{{table}}
{|{{table}}

Revision as of 02:37, 25 February 2014

Cell density and DE differentiation

Setting: Expose 1000 ms; PFBH 406, 10x obj, SOX17/DAPI

1x seeding density 3x seeding density 10x seeding density
File:022214 H9DE 1x SOX17.png File:022214 H9DE 3x SOX17.png File:022214 H9DE 10x SOX17.png
File:022214 H9DE 1x DAPI.png File:022214 H9DE 3x DAPI.png File:022214 H9DE 10x DAPI.png

DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS

1x seeding density: D6-D7 hES passage at 1:12-1:15 ratio

High seeding density results in high efficient DE differentiation


Return