Song Chen:Daily/NOTES/2014-2-22: Difference between revisions
Jump to navigation
Jump to search
>Chensong No edit summary |
>Chensong No edit summary |
||
(5 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
===Cell density and DE differentiation=== | ===Cell density and DE differentiation=== | ||
Setting: Expose | Setting: Expose 1000 ms, PFBH 406, 10x obj, | ||
<font color=red>SOX17</font><b>/</b><font color=blue>DAPI</font> | |||
{|{{table}} | {|{{table}} | ||
|<b>1x seeding density</b> | |<b>1x seeding density</b> | ||
Line 15: | Line 16: | ||
|} | |} | ||
DE differentiation condition: D1 | DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS | ||
1x seeding density: D6-D7 hES passage at 1:12-1:15 ratio | 1x seeding density: D6-D7 hES passage at 1:12-1:15 ratio |
Latest revision as of 02:37, 25 February 2014
Cell density and DE differentiation[edit]
Setting: Expose 1000 ms, PFBH 406, 10x obj, SOX17/DAPI
1x seeding density | 3x seeding density | 10x seeding density |
File:022214 H9DE 1x SOX17.png | File:022214 H9DE 3x SOX17.png | File:022214 H9DE 10x SOX17.png |
File:022214 H9DE 1x DAPI.png | File:022214 H9DE 3x DAPI.png | File:022214 H9DE 10x DAPI.png |
DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS
1x seeding density: D6-D7 hES passage at 1:12-1:15 ratio
High seeding density results in high efficient DE differentiation