Song Chen:Daily/NOTES/2014-2-21: Difference between revisions

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(Created page with "===Hepatic commitment from high-density DE differentiation=== Setting: Expose 1000 ms, PFBH 406, 10x obj, <font color=green>AFP</font><b>/</b><font color=red>HNF4A</font>; <fo...")
 
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===Hepatic commitment from high-density DE differentiation===
===Hepatic commitment from high-density DE differentiation===
Setting: Expose 1000 ms, PFBH 406, 10x obj,
Setting: Expose 1000 ms, PFBH 406, 10x obj,
<font color=green>AFP</font><b>/</b><font color=red>HNF4A</font>;
<font color=green>AFP</font><b>/</b><font color=red>HNF6</font>;
<font color=green>AFP</font><b>/</b><font color=red>PROX1</font>;


{|{{table}}
{|{{table}}
|<b><font color=green>AFP</font><b>/</b><font color=red>HNF4A</font></b>
|<b><font color=green>AFP</font>/<font color=red>HNF4A</font></b>
|<b><font color=green>AFP</font><b>/</b><font color=red>HNF6</font></b>
|<b><font color=green>AFP</font>/<font color=red>HNF6</font></b>
|<b><font color=green>AFP</font><b>/</b><font color=red>PROX1</font></b>
|<b><font color=green>AFP</font>/<font color=red>PROX1</font></b>
|-
|-
|[[File:022114_H9DE_AFP.png|300px]]
|[[File:022114_H9DE_AFP.png|300px]]

Revision as of 23:03, 28 February 2014

Hepatic commitment from high-density DE differentiation

Setting: Expose 1000 ms, PFBH 406, 10x obj,

AFP/HNF4A AFP/HNF6 AFP/PROX1
File:022114 H9DE AFP.png File:022114 H9DE AFP.2.png File:022114 H9DE AFP.3.png
File:022114 H9DE HNF4A.png File:022114 H9DE HNF6.png File:022114 H9DE PROX1.png

DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS

High seeding density: D6-D7 hES passage at 1:1.2-1:1.5 ratio

Successful hepatic differentiation of high-density DE differentiated cells


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