Song Chen:Daily/NOTES/2014-2-21: Difference between revisions
Jump to navigation
Jump to search
>Chensong (Created page with "===Hepatic commitment from high-density DE differentiation=== Setting: Expose 1000 ms, PFBH 406, 10x obj, <font color=green>AFP</font><b>/</b><font color=red>HNF4A</font>; <fo...") |
>Chensong No edit summary |
||
Line 1: | Line 1: | ||
===Hepatic commitment from high-density DE differentiation=== | ===Hepatic commitment from high-density DE differentiation=== | ||
Setting: Expose 1000 ms, PFBH 406, 10x obj, | Setting: Expose 1000 ms, PFBH 406, 10x obj, | ||
{|{{table}} | {|{{table}} | ||
|<b><font color=green>AFP</font | |<b><font color=green>AFP</font>/<font color=red>HNF4A</font></b> | ||
|<b><font color=green>AFP</font | |<b><font color=green>AFP</font>/<font color=red>HNF6</font></b> | ||
|<b><font color=green>AFP</font | |<b><font color=green>AFP</font>/<font color=red>PROX1</font></b> | ||
|- | |- | ||
|[[File:022114_H9DE_AFP.png|300px]] | |[[File:022114_H9DE_AFP.png|300px]] |
Revision as of 23:03, 28 February 2014
Hepatic commitment from high-density DE differentiation
Setting: Expose 1000 ms, PFBH 406, 10x obj,
AFP/HNF4A | AFP/HNF6 | AFP/PROX1 |
File:022114 H9DE AFP.png | File:022114 H9DE AFP.2.png | File:022114 H9DE AFP.3.png |
File:022114 H9DE HNF4A.png | File:022114 H9DE HNF6.png | File:022114 H9DE PROX1.png |
DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS
High seeding density: D6-D7 hES passage at 1:1.2-1:1.5 ratio
Successful hepatic differentiation of high-density DE differentiated cells