Kun:LabNotes/ASE/2008-11-26: Difference between revisions
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m (New page: ==Perform capturing experiment with the E27kV2 probe set== A B PGP1 gDNA (125ng/ul,08/06/08) 4ul 4ul ...) |
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==Perform capturing experiment with the E27kV2 probe set== | ==Perform capturing experiment with the E27kV2 probe set== | ||
A B | A B | ||
PGP1 gDNA ( | PGP1 gDNA (158ng/ul,11/26/08) 4ul 4ul | ||
10X buffer 2ul 2ul | 10X buffer 2ul 2ul | ||
E27KV2 Nicking (38nM) 14ul DpnII (140nM) 8ul | E27KV2 Nicking (38nM) 14ul DpnII (140nM) 8ul | ||
H2O 6ul | H2O 6ul | ||
95C 5min -> 60C | 95C 5min -> cool to 70C at 0.1C/sec -> 70C 6h -> cool to 60C at 0.1C/sec | ||
-> 60C 48h -> add 2ul SLN mix () -> 60C 2h -> 94C 1min -> 37C 1min ] | |||
-> add 2u Exo I&III -> 37C 2h -> 90C 5min -> 4C hold |
Revision as of 00:35, 27 November 2008
Perform capturing experiment with the E27kV2 probe set
A B PGP1 gDNA (158ng/ul,11/26/08) 4ul 4ul 10X buffer 2ul 2ul E27KV2 Nicking (38nM) 14ul DpnII (140nM) 8ul H2O 6ul 95C 5min -> cool to 70C at 0.1C/sec -> 70C 6h -> cool to 60C at 0.1C/sec -> 60C 48h -> add 2ul SLN mix () -> 60C 2h -> 94C 1min -> 37C 1min ] -> add 2u Exo I&III -> 37C 2h -> 90C 5min -> 4C hold