Kun:LabNotes/ASE/2008-11-26: Difference between revisions

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m (New page: ==Perform capturing experiment with the E27kV2 probe set== A B PGP1 gDNA (125ng/ul,08/06/08) 4ul 4ul ...)
 
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==Perform capturing experiment with the E27kV2 probe set==
==Perform capturing experiment with the E27kV2 probe set==
                                       A                    B
                                       A                    B
     PGP1 gDNA (125ng/ul,08/06/08)    4ul                  4ul
     PGP1 gDNA (158ng/ul,11/26/08)    4ul                  4ul
     10X buffer                        2ul                  2ul
     10X buffer                        2ul                  2ul
     E27KV2 Nicking (38nM)            14ul    DpnII (140nM) 8ul
     E27KV2 Nicking (38nM)            14ul    DpnII (140nM) 8ul
     H2O                                                    6ul
     H2O                                                    6ul
     95C 5min -> 60C 72h -> add 2ul SLN mix () -> 60C 2h  
     95C 5min -> cool to 70C at 0.1C/sec -> 70C 6h -> cool to 60C at 0.1C/sec
      -> 94C 1min -> 37C 1min -> add 2u Exo I&III -> 37C 2h -> 94C 5min -> 4C hold
      -> 60C 48h -> add 2ul SLN mix () -> 60C 2h -> 94C 1min -> 37C 1min ]
      -> add 2u Exo I&III -> 37C 2h -> 90C 5min -> 4C hold

Revision as of 00:35, 27 November 2008

Perform capturing experiment with the E27kV2 probe set

                                     A                     B
   PGP1 gDNA (158ng/ul,11/26/08)     4ul                   4ul
   10X buffer                        2ul                   2ul
   E27KV2 Nicking (38nM)            14ul     DpnII (140nM) 8ul
   H2O                                                     6ul
   95C 5min -> cool to 70C at 0.1C/sec -> 70C 6h -> cool to 60C at 0.1C/sec 
      -> 60C 48h -> add 2ul SLN mix () -> 60C 2h -> 94C 1min -> 37C 1min ]
      -> add 2u Exo I&III -> 37C 2h -> 90C 5min -> 4C hold