AlanFung:LabNotes/Rolony/2014-3-5: Difference between revisions
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>Alan6017518 (Created page with "==Secondary Rolony Generation for Padlock Probe Concentration Test==") |
>Alan6017518 |
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==Secondary Rolony Generation for Padlock Probe Concentration Test== | ==Secondary Rolony Generation for Padlock Probe Concentration Test== | ||
===Samples=== | |||
*96 well plate containing NCIH-1975 cells fixed by Hosuk | |||
*Location and save location of 12 position in 96 well plate for the experiment | |||
*High Concentration(100pM)- | |||
====Anneal Padlock Probes for 24 hours=== | |||
#1X Ampligase Buffer | |||
#Various padlock probe concentration | |||
#0.5 U/ul Ampligase | |||
#Incubate @ 45C for 24 hours | |||
#Wrapped in parafilm and put in ziploc with wet towel |
Revision as of 19:51, 5 March 2014
Secondary Rolony Generation for Padlock Probe Concentration Test
Samples
- 96 well plate containing NCIH-1975 cells fixed by Hosuk
- Location and save location of 12 position in 96 well plate for the experiment
- High Concentration(100pM)-
=Anneal Padlock Probes for 24 hours
- 1X Ampligase Buffer
- Various padlock probe concentration
- 0.5 U/ul Ampligase
- Incubate @ 45C for 24 hours
- Wrapped in parafilm and put in ziploc with wet towel