AlanFung:LabNotes/Rolony/2014-3-5: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Alan6017518
>Alan6017518
Line 4: Line 4:
*Location and save location of 12 position in 96 well plate for the experiment
*Location and save location of 12 position in 96 well plate for the experiment
*High Concentration(100pM)-
*High Concentration(100pM)-
====Anneal Padlock Probes for 24 hours===
===Anneal Padlock Probes for 24 hours===
#1X Ampligase Buffer
#1X Ampligase Buffer
#Various padlock probe concentration
#Various padlock probe concentration

Revision as of 19:51, 5 March 2014

Secondary Rolony Generation for Padlock Probe Concentration Test

Samples

  • 96 well plate containing NCIH-1975 cells fixed by Hosuk
  • Location and save location of 12 position in 96 well plate for the experiment
  • High Concentration(100pM)-

Anneal Padlock Probes for 24 hours

  1. 1X Ampligase Buffer
  2. Various padlock probe concentration
  3. 0.5 U/ul Ampligase
  4. Incubate @ 45C for 24 hours
  5. Wrapped in parafilm and put in ziploc with wet towel