AlanFung:LabNotes/Rolony/2014-3-5: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 |
>Alan6017518 |
||
Line 4: | Line 4: | ||
*Location and save location of 12 position in 96 well plate for the experiment | *Location and save location of 12 position in 96 well plate for the experiment | ||
*High Concentration(100pM)- | *High Concentration(100pM)- | ||
===Anneal Padlock Probes for 24 hours=== | |||
#1X Ampligase Buffer | #1X Ampligase Buffer | ||
#Various padlock probe concentration | #Various padlock probe concentration |
Revision as of 19:51, 5 March 2014
Secondary Rolony Generation for Padlock Probe Concentration Test
Samples
- 96 well plate containing NCIH-1975 cells fixed by Hosuk
- Location and save location of 12 position in 96 well plate for the experiment
- High Concentration(100pM)-
Anneal Padlock Probes for 24 hours
- 1X Ampligase Buffer
- Various padlock probe concentration
- 0.5 U/ul Ampligase
- Incubate @ 45C for 24 hours
- Wrapped in parafilm and put in ziploc with wet towel