AlanFung:LabNotes/Rolony/2014-3-5: Difference between revisions
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[[File:ppconcentration_NCHI1975_Group1.jpg| | [[File:ppconcentration_NCHI1975_Group1.jpg|1000px]] | ||
====Group2 F8(15), F5(14), F6(7), E6(8)==== | ====Group2 F8(15), F5(14), F6(7), E6(8)==== | ||
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[[File:ppconcentration_NCHI1975_Group2.jpg| | [[File:ppconcentration_NCHI1975_Group2.jpg|1000px]] | ||
====Group3 C6(9), C7(10), E7(11), F7(12)==== | ====Group3 C6(9), C7(10), E7(11), F7(12)==== | ||
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[[File:ppconcentration_NCHI1975_Group3.jpg| | [[File:ppconcentration_NCHI1975_Group3.jpg|1000px]] |
Revision as of 22:56, 5 March 2014
Secondary Rolony Generation for Padlock Probe Concentration Test
Samples
- 96 well plate containing NCIH-1975 cells fixed by Hosuk
- Location and save location of 12 position in 96 well plate for the experiment
- High Concentration(100pM)-
Anneal Padlock Probes for 24 hours
- 1X Ampligase Buffer
- Various padlock probe concentration
- 0.5 U/ul Ampligase
- Incubate @ 45C for 24 hours
- Wrapped in parafilm and put in ziploc with wet towel
Group1 C3(1),D3(2), F3(13), C5(4)
Group 1 | ||
Image | Well | Mark & Find |
A | C3 | 1 |
B | D3 | 2 |
C | F3 | 13 |
D | C5 | 4 |
File:Ppconcentration NCHI1975 Group1.jpg
Group2 F8(15), F5(14), F6(7), E6(8)
Group 2 | ||
Image | Well | Mark & Find |
E | F8 | 15 |
F | F5 | 14 |
G | F6 | 7 |
H | E6 | 8 |
File:Ppconcentration NCHI1975 Group2.jpg
Group3 C6(9), C7(10), E7(11), F7(12)
Group 3 | ||
Image | Well | Mark & Find |
I | C6 | 9 |
J | C7 | 10 |
K | E7 | 11 |
L | F7 | 12 |