AlanFung:LabNotes/Rolony/2014-3-5: Difference between revisions

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#Incubate @ 45C for 24 hours
#Incubate @ 45C for 24 hours
#Wrapped in parafilm and put in ziploc with wet towel
#Wrapped in parafilm and put in ziploc with wet towel
====Group1 High Concentration====
====Group1 100pM ppMALAT1====
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
|- style="font-size:12pt;font-weight:bold" align="center"

Revision as of 22:59, 5 March 2014

Secondary Rolony Generation for Padlock Probe Concentration Test

Samples

  • 96 well plate containing NCIH-1975 cells fixed by Hosuk
  • Location and save location of 12 position in 96 well plate for the experiment
  • High Concentration(100pM)-

Anneal Padlock Probes for 24 hours

  1. 1X Ampligase Buffer
  2. Various padlock probe concentration
  3. 0.5 U/ul Ampligase
  4. Incubate @ 45C for 24 hours
  5. Wrapped in parafilm and put in ziploc with wet towel

Group1 100pM ppMALAT1

Group 1
Image Well Mark & Find
A C3 1
B D3 2
C F3 13
D C5 4

File:Ppconcentration NCHI1975 Group1.jpg

Group2 F8(15), F5(14), F6(7), E6(8)

Group 2
Image Well Mark & Find
E F8 15
F F5 14
G F6 7
H E6 8

File:Ppconcentration NCHI1975 Group2.jpg

Group3 C6(9), C7(10), E7(11), F7(12)

Group 3
Image Well Mark & Find
I C6 9
J C7 10
K E7 11
L F7 12

File:Ppconcentration NCHI1975 Group3.jpg