AlanFung:LabNotes/Rolony/2014-3-5: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Alan6017518
>Alan6017518
No edit summary
Line 43: Line 43:
[[File:ppconcentration_NCHI1975_Group1.jpg|1000px]]
[[File:ppconcentration_NCHI1975_Group1.jpg|1000px]]


====Group 1 10pM ppMALAT1====
====Group 2 10pM ppMALAT1====
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
|- style="font-size:12pt;font-weight:bold" align="center"
Line 76: Line 76:
[[File:ppconcentration_NCHI1975_Group2.jpg|1000px]]
[[File:ppconcentration_NCHI1975_Group2.jpg|1000px]]


====Group 1 0.1pM ppMALAT1====
====Group 3 0.1pM ppMALAT1====
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
|- style="font-size:12pt;font-weight:bold" align="center"

Revision as of 23:01, 5 March 2014

Secondary Rolony Generation for Padlock Probe Concentration Test

Samples

  • 96 well plate containing NCIH-1975 cells fixed by Hosuk
  • Location and save location of 12 position in 96 well plate for the experiment
  • High Concentration(100pM)-

Anneal Padlock Probes for 24 hours

  1. 1X Ampligase Buffer
  2. Various padlock probe concentration
  3. 0.5 U/ul Ampligase
  4. Incubate @ 45C for 24 hours
  5. Wrapped in parafilm and put in ziploc with wet towel

Group 1 100pM ppMALAT1

Group 1
Image Well Mark & Find
A C3 1
B D3 2
C F3 13
D C5 4

File:Ppconcentration NCHI1975 Group1.jpg

Group 2 10pM ppMALAT1

Group 2
Image Well Mark & Find
E F8 15
F F5 14
G F6 7
H E6 8

File:Ppconcentration NCHI1975 Group2.jpg

Group 3 0.1pM ppMALAT1

Group 3
Image Well Mark & Find
I C6 9
J C7 10
K E7 11
L F7 12

File:Ppconcentration NCHI1975 Group3.jpg