AlanFung:LabNotes/Rolony/2014-3-5: Difference between revisions
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Revision as of 23:06, 5 March 2014
Secondary Rolony Generation for Padlock Probe Concentration Test
Samples
- 96 well plate containing NCIH-1975 cells fixed by Hosuk
- Location and save location of 12 position in 96 well plate for the experiment
- High Concentration(100pM)-
Anneal Padlock Probes for 24 hours
- 1X Ampligase Buffer
- Various padlock probe concentration
- 0.5 U/ul Ampligase
- Incubate @ 45C for 24 hours
- Wrapped in parafilm and put in ziploc with wet towel
Group 1 100pM ppMALAT1
Group 1 | ||
Image | Well | Mark & Find |
A | C3 | 1 |
B | D3 | 2 |
C | F3 | 13 |
D | C5 | 4 |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | ||||||||||||
B | ||||||||||||
C | C3 | C5 | C6 | C7 | ||||||||
D | D3 | |||||||||||
E | E6 | E7 | ||||||||||
F | F3 | F5 | F6 | F7 | F8 | |||||||
G | ||||||||||||
H |
File:Ppconcentration NCHI1975 Group1.jpg
Group 2 10pM ppMALAT1
Group 2 | ||
Image | Well | Mark & Find |
E | F8 | 15 |
F | F5 | 14 |
G | F6 | 7 |
H | E6 | 8 |
File:Ppconcentration NCHI1975 Group2.jpg
Group 3 0.1pM ppMALAT1
Group 3 | ||
Image | Well | Mark & Find |
I | C6 | 9 |
J | C7 | 10 |
K | E7 | 11 |
L | F7 | 12 |