AlanFung:LabNotes/Rolony/2014-3-5: Difference between revisions
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====Group 1 100pM ppMALAT1==== | ====Group 1 100pM ppMALAT1==== | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext> | ||
|- style="font-size:12pt;font-weight:bold" align="center" | |- style="font-size:12pt" align="center" | ||
| width="65" colspan="3" | |style="background-color:#FFFF00;font-weight:bold" width="65" colspan="3" height="15" | Group 1 | ||
| align="center" width="65" | | |||
|style="background-color:#9BBB59;font-weight:bold" width="65" colspan="3" | Group 2 | |||
| align="center" width="65" | | |||
|style="background-color:#4F81BD;font-weight:bold" width="65" colspan="3" | Group 3 | |||
|- style="font-size:12pt;font-weight:bold" align="center" | |- style="font-size:12pt" align="center" | ||
| | |style="font-weight:bold" height="15" | Image | ||
|style="font-weight:bold" | Well | |||
|style="font-weight:bold" | Mark & Find | |||
| align="center" | | |||
|style="font-weight:bold" | Image | |||
|style="font-weight:bold" | Well | |||
|style="font-weight:bold" | Mark & Find | |||
| align="center" | | |||
|style="font-weight:bold" | Image | |||
|style="font-weight:bold" | Well | |||
|style="font-weight:bold" | Mark & Find | |||
|- style="font-size:12pt" align="center" | |- style="font-size:12pt" align="center" | ||
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| C3 | | C3 | ||
| align="center" | 1 | | align="center" | 1 | ||
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| I | |||
| C6 | |||
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|} | |} |
Revision as of 23:07, 5 March 2014
Secondary Rolony Generation for Padlock Probe Concentration Test
Samples
- 96 well plate containing NCIH-1975 cells fixed by Hosuk
- Location and save location of 12 position in 96 well plate for the experiment
- High Concentration(100pM)-
Anneal Padlock Probes for 24 hours
- 1X Ampligase Buffer
- Various padlock probe concentration
- 0.5 U/ul Ampligase
- Incubate @ 45C for 24 hours
- Wrapped in parafilm and put in ziploc with wet towel
Group 1 100pM ppMALAT1
Group 1 | Group 2 | Group 3 | ||||||||
Image | Well | Mark & Find | Image | Well | Mark & Find | Image | Well | Mark & Find | ||
A | C3 | 1 | E | F8 | 15 | I | C6 | 9 | ||
B | D3 | 2 | F | F5 | 14 | J | C7 | 10 | ||
C | F3 | 13 | G | F6 | 7 | K | E7 | 11 | ||
D | C5 | 4 | H | E6 | 8 | L | F7 | 12 |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | ||||||||||||
B | ||||||||||||
C | C3 | C5 | C6 | C7 | ||||||||
D | D3 | |||||||||||
E | E6 | E7 | ||||||||||
F | F3 | F5 | F6 | F7 | F8 | |||||||
G | ||||||||||||
H |
File:Ppconcentration NCHI1975 Group1.jpg
Group 2 10pM ppMALAT1
Group 2 | ||
Image | Well | Mark & Find |
E | F8 | 15 |
F | F5 | 14 |
G | F6 | 7 |
H | E6 | 8 |
File:Ppconcentration NCHI1975 Group2.jpg
Group 3 0.1pM ppMALAT1
Group 3 | ||
Image | Well | Mark & Find |
I | C6 | 9 |
J | C7 | 10 |
K | E7 | 11 |
L | F7 | 12 |