Kun:LabNotes/CpgSeq/2007-11-9: Difference between revisions
Jump to navigation
Jump to search
(New page: ===Exp. 1 Perform PCR on the bisulfite converted DNAs (positive control)=== *Template: Jurkat DNA, DNA RL, concentration unknown. *Primer: hd48MIP-1, hd48MIP-2, hd48MIP-5. ...) |
|||
Line 14: | Line 14: | ||
94C 3min -> 40 cycles of (94C 30sec -> 57C 30min -> 72C 1min) -> 72C 3min -> 15C hold. | 94C 3min -> 40 cycles of (94C 30sec -> 57C 30min -> 72C 1min) -> 72C 3min -> 15C hold. | ||
===Exp. 2 Measure the concentration of PAGE purified padlock probes by gel electrophoresis=== | |||
Set 1: 3.7ng/ul = 128nM | |||
Set 2: 4.2ng/ul = 162nM |
Revision as of 01:31, 10 November 2007
Exp. 1 Perform PCR on the bisulfite converted DNAs (positive control)
- Template: Jurkat DNA, DNA RL, concentration unknown.
- Primer: hd48MIP-1, hd48MIP-2, hd48MIP-5.
x1 x6 template: 1ul each 10x ABI buffer: 2.5ul 15ul 25mM MgCl2: 1.5ul 9ul 10mM dNTP: 0.5ul 3ul 10uM Primer mix: 0.5ul 1ulx3 50X SYBG I: 0.2ul 1.2ul iTaq: 0.2ul 1.2ul H2O: 18.6ul 114ul
94C 3min -> 40 cycles of (94C 30sec -> 57C 30min -> 72C 1min) -> 72C 3min -> 15C hold.
Exp. 2 Measure the concentration of PAGE purified padlock probes by gel electrophoresis
Set 1: 3.7ng/ul = 128nM Set 2: 4.2ng/ul = 162nM