Song Chen:Daily/NOTES/2014-3-6: Difference between revisions
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<b>check default GFP expression in knockin iPS lines</b>: | <b>check default GFP expression in knockin iPS lines</b>: | ||
Setting: Expose 1209 ms, Cerc, 20x obj, | Setting: Expose 1209 ms, Cerc, 20x obj,<font color=green>GFP</font>/Brightfield, KI(knockin) | ||
{|{{table}} | {|{{table}} | ||
| | |KI#1 | ||
| | |KI#2-4 | ||
| | |KI#2-6 | ||
|Bb#6(random integrated) | |||
|- | |- | ||
|[[File: | |[[File:030614_KI-1_GFP.png|300px]] | ||
|[[File: | |[[File:030614_KI-2-4_GFP.png|300px]] | ||
|[[File: | |[[File:030614_KI-2-6_GFP.png|300px]] | ||
|[[File:030614_Bb6_GFP.png|300px]] | |||
|- | |||
|[[File:030614_KI-1_BF.png|300px]] | |||
|[[File:030614_KI-2-4_BF.png|300px]] | |||
|[[File:030614_KI-2-6_BF.png|300px]] | |||
|[[File:030614_Bb6_BF.png|300px]] | |||
|- | |||
|[[File:030614_KI-1_Merge.png|300px]] | |||
|[[File:030614_KI-2-4_Merge.png|300px]] | |||
|[[File:030614_KI-2-6_Merge.png|300px]] | |||
|[[File:030614_Bb6_Merge.png|300px]] | |||
|} | |} |
Revision as of 22:05, 7 March 2014
hES change medium:
- PGP iPS Brainbow knockin #1, #2-4 and #2-6 p2, 2 well*6 well plate in hES/NHSM, respectively;
- H9 p62 2*6 cm+24 well*48-well plate in NHSM
- H9 Bb6 2 well*6 well plate
- PGPs iPS LCB+CRE p62 1*6 cm in hES medium
send H9 in NHSM:
- 1*T25 flask
DE differentition(H9 p62, for methylation analysis-Dinh):
- 3 well*6-well plate, hES at d2, ActA100 d4
check default GFP expression in knockin iPS lines: Setting: Expose 1209 ms, Cerc, 20x obj,GFP/Brightfield, KI(knockin)