Song Chen:Daily/NOTES/2014-3-6: Difference between revisions

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|Bb#6(random integrated)
|Bb#6(random integrated)
|-
|-
|[[File:030614_KI-1_GFP.png|300px]]
|[[File:030614_KI-1_GFP.png|200px]]
|[[File:030614_KI-2-4_GFP.png|300px]]
|[[File:030614_KI-2-4_GFP.png|200px]]
|[[File:030614_KI-2-6_GFP.png|300px]]
|[[File:030614_KI-2-6_GFP.png|200px]]
|[[File:030614_Bb6_GFP.png|300px]]
|[[File:030614_Bb6_GFP.png|200px]]
|-
|-
|[[File:030614_KI-1_BF.png|300px]]
|[[File:030614_KI-1_BF.png|200px]]
|[[File:030614_KI-2-4_BF.png|300px]]
|[[File:030614_KI-2-4_BF.png|200px]]
|[[File:030614_KI-2-6_BF.png|300px]]
|[[File:030614_KI-2-6_BF.png|200px]]
|[[File:030614_Bb6_BF.png|300px]]
|[[File:030614_Bb6_BF.png|200px]]
|-
|-
|[[File:030614_KI-1_Merge.png|300px]]
|[[File:030614_KI-1_Merge.png|200px]]
|[[File:030614_KI-2-4_Merge.png|300px]]
|[[File:030614_KI-2-4_Merge.png|200px]]
|[[File:030614_KI-2-6_Merge.png|300px]]
|[[File:030614_KI-2-6_Merge.png|200px]]
|[[File:030614_Bb6_Merge.png|300px]]
|[[File:030614_Bb6_Merge.png|200px]]
|}
|}

Revision as of 22:11, 7 March 2014

hES change medium:

  1. PGP iPS Brainbow knockin #1, #2-4 and #2-6 p2, 2 well*6 well plate in hES/NHSM, respectively;
  2. H9 p62 2*6 cm+24 well*48-well plate in NHSM
  3. H9 Bb6 2 well*6 well plate
  4. PGPs iPS LCB+CRE p62 1*6 cm in hES medium

send H9 in NHSM:

1*T25 flask


DE differentition(H9 p62, for methylation analysis-Dinh):

3 well*6-well plate, hES at d2, ActA100 d4

check default GFP expression in knockin iPS lines: Setting: Expose 1209 ms, Cerc, 20x obj,GFP/Brightfield, KI(knockin)

KI#1 KI#2-4 KI#2-6 Bb#6(random integrated)
File:030614 KI-1 GFP.png File:030614 KI-2-4 GFP.png File:030614 KI-2-6 GFP.png File:030614 Bb6 GFP.png
File:030614 KI-1 BF.png File:030614 KI-2-4 BF.png File:030614 KI-2-6 BF.png File:030614 Bb6 BF.png
File:030614 KI-1 Merge.png File:030614 KI-2-4 Merge.png File:030614 KI-2-6 Merge.png File:030614 Bb6 Merge.png