Dinh/Dinh 2014/NOTES/2014-3-11: Difference between revisions
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| Amount Probes needed||1.69||ng | | Amount Probes needed||1.69||ng | ||
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Human MW = ( | Human MW = (3x10<sup>9</sup> bp)*(607.4 gmoles<sup>-1</sup>/bp)+157.9gmoles<sup>-1</sup> | ||
1 probe MW = (103 bp)*(303.7 gmoles<sup>-1</sup>)+ 79 gmoles<sup>-1</sup> | |||
Amount probes = (1 probe MW) * (Size of probes) * (Moles of probes required) |
Revision as of 16:46, 19 March 2014
HOTSPOTS 460K Capture
- Capture calculations
Size of Probes | 90000 | units |
gDNA | 150 | ng |
Human (MW) | 1.82E+012 | g/moles |
1 Probe (MW) | 31360.1 | g/moles |
Template | 8.23E-011 | nmoles |
Probes(100:1) | 8.23E-009 | nmoles |
Amount Probes needed | 23.23 | ng |
Size of Probes | 6553 | units |
gDNA | 150 | ng |
Human (MW) | 1.82E+012 | g/moles |
1 Probe (MW) | 31360.1 | g/moles |
Template | 8.23E-011 | nmoles |
Probes(100:1) | 8.23E-009 | nmoles |
Amount Probes needed | 1.69 | ng |
Human MW = (3x109 bp)*(607.4 gmoles-1/bp)+157.9gmoles-1 1 probe MW = (103 bp)*(303.7 gmoles-1)+ 79 gmoles-1 Amount probes = (1 probe MW) * (Size of probes) * (Moles of probes required)