Dinh/Dinh 2014/NOTES/2014-3-11: Difference between revisions

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| Amount Probes needed||1.69||ng
| Amount Probes needed||1.69||ng
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   Human MW = (3E109 bp)*(607.4 gmoles<sup>-1</sup>/bp)+157.9gmoles<sup>-1</sup>
   Human MW = (3x10<sup>9</sup> bp)*(607.4 gmoles<sup>-1</sup>/bp)+157.9gmoles<sup>-1</sup>
  1 probe MW = (103 bp)*(303.7 gmoles<sup>-1</sup>)+ 79 gmoles<sup>-1</sup>
  Amount probes = (1 probe MW) * (Size of probes) * (Moles of probes required)

Revision as of 16:46, 19 March 2014

HOTSPOTS 460K Capture

  • Capture calculations
Size of Probes 90000 units
gDNA 150 ng
Human (MW) 1.82E+012 g/moles
1 Probe (MW) 31360.1 g/moles
Template 8.23E-011 nmoles
Probes(100:1) 8.23E-009 nmoles
Amount Probes needed 23.23 ng
Size of Probes 6553 units
gDNA 150 ng
Human (MW) 1.82E+012 g/moles
1 Probe (MW) 31360.1 g/moles
Template 8.23E-011 nmoles
Probes(100:1) 8.23E-009 nmoles
Amount Probes needed 1.69 ng
 Human MW = (3x109 bp)*(607.4 gmoles-1/bp)+157.9gmoles-1
 1 probe MW = (103 bp)*(303.7 gmoles-1)+ 79 gmoles-1
 Amount probes = (1 probe MW) * (Size of probes) * (Moles of probes required)