Dinh/Dinh 2014/NOTES/2014-4-8: Difference between revisions
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* Pool sequencing library together, concentration is ~2.64 ng/ul | * Pool sequencing library together, concentration is ~2.64 ng/ul | ||
* Since Indx28 is only 1/5 of the total library (band at 450 bp), and 29,33,35,37 are at 510 bp, the average size is: | * Since Indx28 is only 1/5 of the total library (band at 450 bp), and 29,33,35,37 are at 510 bp, the average size is: | ||
450*0.20 + 510*0.80 = 498 bp | 450*0.20 + 510*0.80 = 498 bp is the average library size. | ||
* TBE Gel: | * TBE Gel: | ||
[[File:HOTSPOTS_seqLib_Apr9.png]] | [[File:HOTSPOTS_seqLib_Apr9.png]] |
Revision as of 17:18, 10 April 2014
HOTSPOTS 460K sequencing library
- Indx 28, Indx 29 gel cut:
File:HOTSPOTS Indx28,Indx29 sizeselect.jpg
- Added three libraries with Index 33,35,37 from full HOTSPOTS 460K capture of GM12878, cut from top band.
- Pool sequencing library together, concentration is ~2.64 ng/ul
- Since Indx28 is only 1/5 of the total library (band at 450 bp), and 29,33,35,37 are at 510 bp, the average size is:
450*0.20 + 510*0.80 = 498 bp is the average library size.
- TBE Gel:
File:HOTSPOTS seqLib Apr9.png