Dinh/Dinh 2014/NOTES/2014-4-8: Difference between revisions
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>Dinh (Created page with "= HOTSPOTS 460K sequencing library = * Indx 28, Indx 29 gel cut: * Added three libraries with Index 33,35,37 from full HOTSPOTS 460K capture of GM12878, cut from top band. ...") |
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= HOTSPOTS 460K sequencing library = | = HOTSPOTS 460K sequencing library = | ||
* Indx 28, Indx 29 gel cut: | * Indx 28, Indx 29 gel cut: (Indx28 and 29 are only captured using subset C) | ||
[[File:HOTSPOTS_Indx28,Indx29_sizeselect.jpg | 300px]] | |||
* Added three libraries with Index 33,35,37 from full HOTSPOTS 460K capture of GM12878, cut from top band. | * Added three libraries with Index 33,35,37 from full HOTSPOTS 460K capture of GM12878, cut from top band. | ||
* Pool sequencing library together, concentration is ~2.64 ng/ul | * Pool sequencing library together, concentration is ~2.64 ng/ul | ||
* Since Indx28 is only 1/5 of the total library (band at 450 bp), and 29,33,35,37 are at 510 bp, the average size is: | |||
450*0.20 + 510*0.80 = 498 bp is the average library size. | |||
* Library name: '''DD-BSPP_HOTSPOTS-GM12878-Apr9''' | |||
* TBE Gel: | * TBE Gel: | ||
[[File:HOTSPOTS_seqLib_Apr9.png]] | [[File:HOTSPOTS_seqLib_Apr9.png]] |
Latest revision as of 17:24, 10 April 2014
HOTSPOTS 460K sequencing library[edit]
- Indx 28, Indx 29 gel cut: (Indx28 and 29 are only captured using subset C)
File:HOTSPOTS Indx28,Indx29 sizeselect.jpg
- Added three libraries with Index 33,35,37 from full HOTSPOTS 460K capture of GM12878, cut from top band.
- Pool sequencing library together, concentration is ~2.64 ng/ul
- Since Indx28 is only 1/5 of the total library (band at 450 bp), and 29,33,35,37 are at 510 bp, the average size is:
450*0.20 + 510*0.80 = 498 bp is the average library size.
- Library name: DD-BSPP_HOTSPOTS-GM12878-Apr9
- TBE Gel:
File:HOTSPOTS seqLib Apr9.png