Kun:LabNotes/ASE/2007-11-13: Difference between revisions

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(New page: ===Exp.2 eSNPs capture (cont. on the 2007-11-11 exp.)=== Perform PCR on 5ul capturing reactions (100ul for gDNA). x 1 x 12 template: 5ul...)
 
Line 13: Line 13:
       H2O:              35.3ul        430ul
       H2O:              35.3ul        430ul


94C 3min -> 10 cycles of (94C 45sec -> 56C 2min -> 72C 1min) -> 20 cycles of (94C 45 sec -> 72C 1min) -> 72C 3min -> 15C hold.
94C 3min -> 10 cycles of (94C 45sec -> 56C 2min -> 72C 1min) -> 12 cycles of (94C 45 sec -> 72C 1min) -> 72C 3min -> 15C hold.

Revision as of 23:04, 13 November 2007

Exp.2 eSNPs capture (cont. on the 2007-11-11 exp.)

Perform PCR on 5ul capturing reactions (100ul for gDNA).

                          x 1        x 12
     template:            5ul         each
     ABI 10x buffer:    4.5ul         54ul
     25mM MgCl2:          3ul         36ul
     10mM dNTP:           1ul         12ul
     100uM AmpF2Sol:   0.15ul        1.8ul
     100uM AmpRSol:    0.15ul        1.8ul
     50X SYBG I:        0.4ul        4.8ul
     iTaq:              0.5ul          6ul
     H2O:              35.3ul        430ul

94C 3min -> 10 cycles of (94C 45sec -> 56C 2min -> 72C 1min) -> 12 cycles of (94C 45 sec -> 72C 1min) -> 72C 3min -> 15C hold.