Noi/NOTES/2014-6-1: Difference between revisions
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>Noi (Created page with "= MONOD V1N3 BSPP capture (repeat sensitivity test) = '''Link to calendar: http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes#2014''' * I repeated MONOD V...") |
>Noi |
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* Loaded 4ul of PCR products in 6% TBE gel | * Loaded 4ul of PCR products in 6% TBE gel | ||
[[File:ZhangLab_2 2014-06-03 16hr 20min_V1N3_500to1.jpg| 450px]] <br> | [[File:ZhangLab_2 2014-06-03 16hr 20min_V1N3_500to1.jpg| 450px]] <br> | ||
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[[File:ZhangLab_2 2014-06-03 16hr 20min_V1N3_1000to1.jpg| 450px]] | [[File:ZhangLab_2 2014-06-03 16hr 20min_V1N3_1000to1.jpg| 450px]] |
Revision as of 06:33, 4 June 2014
MONOD V1N3 BSPP capture (repeat sensitivity test)
Link to calendar: [[1]]
- I repeated MONOD V1N3 capture experiment on 3014-05-29.
- I reduced capture volume to 10ul.
- I reduced incubation time after adding SLN mix between 4-6h since I think that 20h is not necessary for polymerization and may cause more back ground if DNA template level is too low.
- I will add experimental procedures later
PAGE verification
- Loaded 4ul of PCR products in 6% TBE gel
File:ZhangLab 2 2014-06-03 16hr 20min V1N3 500to1.jpg
File:ZhangLab 2 2014-06-03 16hr 20min V1N3 1000to1.jpg