Noi/NOTES/2014-6-3: Difference between revisions

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* Performed PAGE size selection in 3 wells of 5-well 12% TBE gel
* Performed PAGE size selection in 3 wells of 5-well 12% TBE gel
  [[File:ZhangLab_2 2014-06-06 10hr 08min_5ng-CTT-RRBS_PAGE-SS.jpg| 400px]]
  [[File:ZhangLab_2 2014-06-06 10hr 08min_5ng-CTT-RRBS_PAGE-SS.jpg| 400px]]
* Verified 2ul of size-selected libraries in 6% TBE gel
[[File:ZhangLab_2 2014-06-07 22hr 50min_PAGE-verify_NP-RRBS-CTT-5ng.jpg|150px]]
'''Sequencing library ID: NP-RRBS-CTT-5ng, Jun3, 2014'''
'''Sequencing library ID: NP-RRBS-CTT-5ng, Jun3, 2014'''
:- Length: 160-360bp
:- Length: 160-360bp
:- Index: Truseq Ind9-11, 13
:- Index: Truseq Ind9-11, 13

Revision as of 14:23, 8 June 2014

RRBS, tumor tissue sample (colon)

Link to calendar: [[1]]

  • Experimental procedur ewill be added
PAGE verification
  • Loaded 4ul of 1X bead-purified sequencing libraries (total 60ul) in 12% TBE gel
File:ZhangLab 2 2014-06-06 08hr 41min PQ 5ng-CTT-RRBS 4ul.jpg
PAGE size selection
  • Performed PAGE size selection in 3 wells of 5-well 12% TBE gel
File:ZhangLab 2 2014-06-06 10hr 08min 5ng-CTT-RRBS PAGE-SS.jpg
  • Verified 2ul of size-selected libraries in 6% TBE gel
File:ZhangLab 2 2014-06-07 22hr 50min PAGE-verify NP-RRBS-CTT-5ng.jpg

Sequencing library ID: NP-RRBS-CTT-5ng, Jun3, 2014

- Length: 160-360bp
- Index: Truseq Ind9-11, 13