Daniel:Notebook/HiResChrPaint/2014-6-16: Difference between revisions

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Using the Oligopaints scripts by Beliveau, my goal is to design a positive/negative control for the FISH probes.  The control is to focus on a region of the X chromosome, yet to be determined.  Using male cells (such as PGP1), exactly one region should be painted per cell.  This serves as a potential positive and negative control.
Using the Oligopaints scripts by Beliveau, my goal is to design a positive/negative control for the FISH probes.  The control is to focus on a region of the X chromosome, yet to be determined.  Using male cells (such as PGP1), exactly one region should be painted per cell.  This serves as a potential positive and negative control.
==Probe Mining==
'''Scripts:'''
[[Media:Oligopaints_Scripts_Manual.pdf‎|Oligopaints Scripts Manual]]
[[Media:]]
Setting up the scripts for probe mining.  I'm doing this locally on my machine for now.
===Setting up a BLAST Database===
Required for running the Oligopaints scripts.

Revision as of 22:56, 16 June 2014

Probe Design

Back to Calendar

Using the Oligopaints scripts by Beliveau, my goal is to design a positive/negative control for the FISH probes. The control is to focus on a region of the X chromosome, yet to be determined. Using male cells (such as PGP1), exactly one region should be painted per cell. This serves as a potential positive and negative control.

Probe Mining

Scripts: Oligopaints Scripts Manual [[Media:]]


Setting up the scripts for probe mining. I'm doing this locally on my machine for now.

Setting up a BLAST Database

Required for running the Oligopaints scripts.