Kun:LabNotes/Haplotyping: Difference between revisions
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==Project threads== | |||
*Regular MDA on diluted chromosomes. | |||
**[[Kun:LabNotes/Haplotyping/2010-8-18|Chromosome prep]] | |||
**[[Kun:LabNotes/Haplotyping/2010-8-19|MDA]] | |||
**[[Kun:LabNotes/Haplotyping/2010-8-26|Library Prep & sequencing]] | |||
**Major conclusions: | |||
***Amplification from diluted chromosomes is an viable option. | |||
***The chromosome concentration was over-estimated by roughly three folds. | |||
***Compared with purified gDNA, the amplification curves on chromosomes tend to shoot up earlier. Therefore, we can't use the amplification curves on purified gDNA to estimate the number of chromosomes in each reaction. | |||
***Three chromosomes were present in the Amplicon 1A (small chromosomes, 10 chr/reaction). It is very likely that the other three amplicons (1B,1C,1D) from the same batch are good too. | |||
==Daily Notes== | |||
2010 | 2010 | ||
<calendar> | <calendar> |
Revision as of 00:46, 18 September 2010
Project threads
- Regular MDA on diluted chromosomes.
- Chromosome prep
- MDA
- Library Prep & sequencing
- Major conclusions:
- Amplification from diluted chromosomes is an viable option.
- The chromosome concentration was over-estimated by roughly three folds.
- Compared with purified gDNA, the amplification curves on chromosomes tend to shoot up earlier. Therefore, we can't use the amplification curves on purified gDNA to estimate the number of chromosomes in each reaction.
- Three chromosomes were present in the Amplicon 1A (small chromosomes, 10 chr/reaction). It is very likely that the other three amplicons (1B,1C,1D) from the same batch are good too.
Daily Notes
2010 <calendar> name=Kun:LabNotes/Haplotyping format=%name/%year-%month-%day date=2010/02/01 view=oneyear </calendar> 2009 <calendar> name=Kun:LabNotes/Haplotyping format=%name/%year-%month-%day date=2009/02/01 view=oneyear </calendar> Protocols