Kun:LabNotes/Haplotyping: Difference between revisions

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==Project threads==
==Project threads==
*Regular MDA on diluted chromosomes.
#Regular MDA on diluted chromosomes(Aug-Sept, 2010)
**[[Kun:LabNotes/Haplotyping/2010-8-18|Chromosome prep]]
##[[Kun:LabNotes/Haplotyping/2010-8-18|Chromosome prep]]
**[[Kun:LabNotes/Haplotyping/2010-8-19|MDA]]
##[[Kun:LabNotes/Haplotyping/2010-8-19|MDA]]
**[[Kun:LabNotes/Haplotyping/2010-8-26|Library Prep & sequencing]]
##[[Kun:LabNotes/Haplotyping/2010-8-26|Library Prep & sequencing]]
**Major conclusions:
##[[Kun:LabNotes/Haplotyping/2010-9-17|Data analysis]]
***Amplification from diluted chromosomes is an viable option.
 
***The chromosome concentration was over-estimated by roughly three folds.
***Compared with purified gDNA, the amplification curves on chromosomes tend to shoot up earlier. Therefore, we can't use the amplification curves on purified gDNA to estimate the number of chromosomes in each reaction.
***Three chromosomes were present in the Amplicon 1A (small chromosomes, 10 chr/reaction). It is very likely that the other three amplicons (1B,1C,1D) from the same batch are good too.
==Daily Notes==
==Daily Notes==
2013
<calendar>
name=Kun:LabNotes/Haplotyping
format=%name/%year-%month-%day
date=2013/02/01
view=oneyear
</calendar>
2011
<calendar>
name=Kun:LabNotes/Haplotyping
format=%name/%year-%month-%day
date=2011/02/01
view=oneyear
</calendar>
2010
2010
<calendar>
<calendar>

Latest revision as of 22:13, 7 May 2013

Project threads[edit]

  1. Regular MDA on diluted chromosomes(Aug-Sept, 2010)
    1. Chromosome prep
    2. MDA
    3. Library Prep & sequencing
    4. Data analysis

Daily Notes[edit]

2013 <calendar> name=Kun:LabNotes/Haplotyping format=%name/%year-%month-%day date=2013/02/01 view=oneyear </calendar> 2011 <calendar> name=Kun:LabNotes/Haplotyping format=%name/%year-%month-%day date=2011/02/01 view=oneyear </calendar> 2010 <calendar> name=Kun:LabNotes/Haplotyping format=%name/%year-%month-%day date=2010/02/01 view=oneyear </calendar> 2009 <calendar> name=Kun:LabNotes/Haplotyping format=%name/%year-%month-%day date=2009/02/01 view=oneyear </calendar> Protocols