Daniel:Notebook/HiResChrPaint/2014-7-9: Difference between revisions

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[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]


==DpnII Digest ([[Daniel:Notebook/HiResChrPaint/2014-7-8#DpnII Digest|from 07/08/2014]])==
==DpnII Digest ([[Daniel:Notebook/HiResChrPaint/2014-7-8#DpnII Digestion|from 07/08/2014]])==


#[[Daniel:Protocols/Qiaquick Column|Column Purification]], elute with 40 uL
#[[Daniel:Protocols/Qiaquick Column|Column Purification]], elute with 40 uL

Revision as of 20:09, 9 July 2014

Strand Control (Started 07/08/2014)

Back to Calendar

DpnII Digest (from 07/08/2014)

  1. Column Purification, elute with 40 uL
  2. Measure concentration in nanodrop

Nanodrop Results

Lambda Exonuclease Digestion

  1. Reaction Table


  1. Incubate for 1 hr at 37C
  2. Heat inactivate for 10 minutes at 75C
  3. Purify with Zymo column, elute with 20 uL nfH2O