Daniel:Notebook/HiResChrPaint/2014-7-9: Difference between revisions
Jump to navigation
Jump to search
>Djacobse |
>Djacobse |
||
Line 3: | Line 3: | ||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | ||
==DpnII Digest ([[Daniel:Notebook/HiResChrPaint/2014-7-8#DpnII | ==DpnII Digest ([[Daniel:Notebook/HiResChrPaint/2014-7-8#DpnII Digestion|from 07/08/2014]])== | ||
#[[Daniel:Protocols/Qiaquick Column|Column Purification]], elute with 40 uL | #[[Daniel:Protocols/Qiaquick Column|Column Purification]], elute with 40 uL |
Revision as of 20:09, 9 July 2014
Strand Control (Started 07/08/2014)
DpnII Digest (from 07/08/2014)
- Column Purification, elute with 40 uL
- Measure concentration in nanodrop
Nanodrop Results
Lambda Exonuclease Digestion
- Reaction Table
- Incubate for 1 hr at 37C
- Heat inactivate for 10 minutes at 75C
- Purify with Zymo column, elute with 20 uL nfH2O