Daniel:Notebook/HiResChrPaint/2014-7-9: Difference between revisions
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===Nanodrop Results=== | ===Nanodrop Results=== | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt;font-weight:bold" | |||
| width="65" height="15" valign="bottom" | Sample | |||
| width="65" align="center" valign="bottom" | V6S2AB | |||
| width="65" align="center" valign="bottom" | V6S2CD | |||
| width="65" align="center" valign="bottom" | V6S3AB | |||
| width="65" align="center" valign="bottom" | V6S3CD | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | ng/uL | |||
| align="center" align="center" valign="bottom" | 122.4 | |||
| align="center" align="center" valign="bottom" | 110.7 | |||
| align="center" align="center" valign="bottom" | 137 | |||
| align="center" align="center" valign="bottom" | 133.5 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | ug in 40 uL | |||
| align="center" align="center" valign="bottom" | 4.9 | |||
| align="center" align="center" valign="bottom" | 4.4 | |||
| align="center" align="center" valign="bottom" | 5.5 | |||
| align="center" align="center" valign="bottom" | 5.3 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="15" valign="bottom" | ug total | |||
| align="center" align="center" valign="bottom" | | |||
|style="font-weight:bold" align="center" align="center" valign="bottom" | 9.3 | |||
| align="center" align="center" valign="bottom" | | |||
|style="font-weight:bold" align="center" align="center" valign="bottom" | 10.8 | |||
|- style="font-size:12pt" | |||
|style="font-weight:bold" height="30" valign="bottom" | Yield (Max ~80%) | |||
| align="center" valign="bottom" | | |||
|style="font-weight:bold" align="center" align="center" valign="bottom" | 71.18 | |||
| align="center" align="center" valign="bottom" | | |||
|style="font-weight:bold" align="center" align="center" valign="bottom" | 71.18 | |||
|} | |||
Scary that both of the columns yielded the same percent (I double checked the math). As a note, taking into account that the theoretical maximum yield is 80% (since we remove 20bp out of 100 on each strand), this is approximately a 90% yield from the column. | |||
==Lambda Exonuclease Digestion== | ==Lambda Exonuclease Digestion== |
Revision as of 20:13, 9 July 2014
Strand Control (Started 07/08/2014)
DpnII Digest (from 07/08/2014)
- Column Purification, elute with 40 uL
- Measure concentration in nanodrop
Nanodrop Results
Sample | V6S2AB | V6S2CD | V6S3AB | V6S3CD |
ng/uL | 122.4 | 110.7 | 137 | 133.5 |
ug in 40 uL | 4.9 | 4.4 | 5.5 | 5.3 |
ug total | 9.3 | 10.8 | ||
Yield (Max ~80%) | 71.18 | 71.18 |
Scary that both of the columns yielded the same percent (I double checked the math). As a note, taking into account that the theoretical maximum yield is 80% (since we remove 20bp out of 100 on each strand), this is approximately a 90% yield from the column.
Lambda Exonuclease Digestion
- Reaction Table
- Incubate for 1 hr at 37C
- Heat inactivate for 10 minutes at 75C
- Purify with Zymo column, elute with 20 uL nfH2O