Daniel:Notebook/HiResChrPaint/2014-7-9: Difference between revisions

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>Djacobse
>Djacobse
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===Nanodrop Results===
===Nanodrop Results===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold"
| width="65" height="15"  valign="bottom" | Sample
| width="65" align="center" valign="bottom" | V6S2AB
| width="65" align="center" valign="bottom" | V6S2CD
| width="65" align="center" valign="bottom" | V6S3AB
| width="65" align="center" valign="bottom" | V6S3CD
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | ng/uL
| align="center" align="center" valign="bottom" | 122.4
| align="center" align="center" valign="bottom" | 110.7
| align="center" align="center" valign="bottom" | 137
| align="center" align="center" valign="bottom" | 133.5
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | ug in 40 uL
| align="center" align="center" valign="bottom" | 4.9
| align="center" align="center" valign="bottom" | 4.4
| align="center" align="center" valign="bottom" | 5.5
| align="center" align="center" valign="bottom" | 5.3
|- style="font-size:12pt"
|style="font-weight:bold" height="15"  valign="bottom" | ug total
| align="center" align="center" valign="bottom" |
|style="font-weight:bold" align="center" align="center" valign="bottom" | 9.3
| align="center" align="center" valign="bottom" |
|style="font-weight:bold" align="center" align="center" valign="bottom" | 10.8
|- style="font-size:12pt"
|style="font-weight:bold" height="30"  valign="bottom" | Yield (Max ~80%)
| align="center"  valign="bottom" | &nbsp;
|style="font-weight:bold" align="center" align="center" valign="bottom" | 71.18
| align="center" align="center" valign="bottom" | &nbsp;
|style="font-weight:bold" align="center" align="center" valign="bottom" | 71.18
|}
Scary that both of the columns yielded the same percent (I double checked the math).  As a note, taking into account that the theoretical maximum yield is 80% (since we remove 20bp out of 100 on each strand), this is approximately a 90% yield from the column.


==Lambda Exonuclease Digestion==
==Lambda Exonuclease Digestion==

Revision as of 20:13, 9 July 2014

Strand Control (Started 07/08/2014)

Back to Calendar

DpnII Digest (from 07/08/2014)

  1. Column Purification, elute with 40 uL
  2. Measure concentration in nanodrop

Nanodrop Results

Sample V6S2AB V6S2CD V6S3AB V6S3CD
ng/uL 122.4 110.7 137 133.5
ug in 40 uL 4.9 4.4 5.5 5.3
ug total 9.3 10.8
Yield (Max ~80%)   71.18   71.18

Scary that both of the columns yielded the same percent (I double checked the math). As a note, taking into account that the theoretical maximum yield is 80% (since we remove 20bp out of 100 on each strand), this is approximately a 90% yield from the column.

Lambda Exonuclease Digestion

  1. Reaction Table


  1. Incubate for 1 hr at 37C
  2. Heat inactivate for 10 minutes at 75C
  3. Purify with Zymo column, elute with 20 uL nfH2O