Hosuk:LabNotes/2014-7-11: Difference between revisions

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[[File:PISA_Param_Prev_2014-07-11.png|300px]]
[[File:PISA_Param_Prev_2014-07-11.png|200px]]
[[File:PISA_Param_New_2014-07-11.png|300px]]
[[File:PISA_Param_New_2014-07-11.png|200px]]




[[File:PISA_Param_CountCompare_Prev_New_2014-07-11.png|1100px]]
[[File:PISA_Param_CountCompare_Prev_New_2014-07-11.png|1100px]]

Revision as of 05:34, 16 July 2014

PISA Parameter - Update!

  • According to Jay’s Science paper, the size of DNA nanoball (Rolony) is 200~400nm.
  • 1 pixel size
    • 20x, Zoom = 1, 2048 x 2048 --> 284nm
    • 63x, Zoom = 1, 2048 x 2048 --> 90.14nm
    • 63x, Zoom = 4, 2048 x 2048 --> 22.54nm


  • Currently used area_lower, area_upper
    • 20x obj. image : 4, 50 (2 x 2 px ~ 7 x 7px --> 568nm x 568 nm ~ 2um x 2um)
    • 63x obj. image : 16, 200 (4 x 4 px ~ 14 x 14px --> 360nm x 360 nm ~ 1.26um x 1.26um)
    • 63x obj. image : 64, 800 (8 x 8 px ~ 29 x 29px --> 180nm x 180 nm ~ 642nm x 642nm)


  • Need to be adjusted!
  • Size for Rolony identification --> 300nm ~ 800nm in diameter
    • (Image resolution need to be 4k x 4k, so that 1px size of 20x obj. becomes 142nm x 142nm. But for now, keep the resolution 2k x 2k!)
  • 20x obj. image : 3 ~ 16 (~2 x ~2 px ~ 4 x 4 px --> ~426nm x ~426nm ~ 1.1um x 1.1um
  • 63x obj. image : 9 ~ 81 (3 x 3 px ~ 9 x 9 px --> ~270nm x 270nm ~ 811nm x 811nm
  • 63x obj. image : 100 ~ 1296 (10 x 10 px ~ 36 x 36 px --> 225nm x 225nm ~ 811nm x 811nm


Result

  • 20x, 63x Zoom=4 images decreased rolony count a little
  • But, 63x images increased rolony count a lot…is this real?


File:PISA Param Prev 2014-07-11.png File:PISA Param New 2014-07-11.png


File:PISA Param CountCompare Prev New 2014-07-11.png