Hosuk:LabNotes/2014-7-15: Difference between revisions

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===20x obj. 4096 x 4096 image analysis===
===20x obj. 4096 x 4096 image analysis===
**[[Hosuk:LabNotes/2014-7-11|refer:07/11]]
*1 pixel size = 141.91nm x 141.91nm
*1 pixel size = 141.91nm x 141.91nm


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**area lower: 6
**area lower: 6
**axratio lower: 0.5
**axratio lower: 0.5
**circ upper: 1.6
**circ lower: 0.8
**perim conn: 8
**bkgmult lower: 4
*PISA Param for 20x, 2048 x 2048
**Gaussian Std : 2
**Gaussian Upper : -2e-4
**area upper: 16
**area lower: 3
**axratio lower: 0.5
**circ upper: 1.6
**circ lower: 0.8
**perim conn: 8
**bkgmult lower: 4
*PISA Param for 63x, 2048 x 2048
**Gaussian Std : 3
**Gaussian Upper : -2e-4
**area upper: 81
**area lower: 9
**axratio lower: 0.6
**circ upper: 1.6
**circ lower: 0.8
**perim conn: 8
**bkgmult lower: 4
*PISA Param for 63x Zoom 4, 2048 x 2048
**Gaussian Std : 10
**Gaussian Upper : -3e-4
**area upper: 1296
**area lower: 100
**axratio lower: 0.6
**circ upper: 1.6
**circ upper: 1.6
**circ lower: 0.8
**circ lower: 0.8
**perim conn: 8
**perim conn: 8
**bkgmult lower: 4
**bkgmult lower: 4

Revision as of 16:41, 16 July 2014

20x obj. 4096 x 4096 image analysis

  • area_lower, area_upper : 6 ~ 50 (2.45 x 2.45 px ~ 7 x 7 px --> 347.68nm x 347.68nm ~ 994nm x 994nm)
  • PISA Param for 20x, 4096 x 4096
    • Gaussian Std : 2
    • Gaussian Upper : -5e-4
    • area upper: 50
    • area lower: 6
    • axratio lower: 0.5
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 4


  • PISA Param for 20x, 2048 x 2048
    • Gaussian Std : 2
    • Gaussian Upper : -2e-4
    • area upper: 16
    • area lower: 3
    • axratio lower: 0.5
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 4


  • PISA Param for 63x, 2048 x 2048
    • Gaussian Std : 3
    • Gaussian Upper : -2e-4
    • area upper: 81
    • area lower: 9
    • axratio lower: 0.6
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 4


  • PISA Param for 63x Zoom 4, 2048 x 2048
    • Gaussian Std : 10
    • Gaussian Upper : -3e-4
    • area upper: 1296
    • area lower: 100
    • axratio lower: 0.6
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 4