Hosuk:LabNotes/2014-7-18: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Hosuki78
No edit summary
>Hosuki78
No edit summary
Line 6: Line 6:
*Generated by '''PSF Generator''' plug-in
*Generated by '''PSF Generator''' plug-in
**Settings
**Settings
[[File:PSFGeneratorSettings_2014-07-18.png.png|1100px]]
[[File:PSFGeneratorSettings_2014-07-18.png|1100px]]





Revision as of 19:43, 1 September 2014

Deconvolution and PSF images

PSF Functions

  • Generated by PSF Generator plug-in
    • Settings

File:PSFGeneratorSettings 2014-07-18.png



Deconvolution

  • Use deconvblind function
    • [img_Decv, PSF] = deconvblind(img_raw, img_PSF);
  • Then do medfilt2, and make img_double


Update PISA Parameter

  • PISA Param for 20x, 2048 x 2048
    • Gaussian Std : 2
    • Gaussian Upper : -2e-4
    • area upper: 16
    • area lower: 3
    • axratio lower: 0.5
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 4


  • PISA Param for 20x, 4096 x 4096
    • Gaussian Std : 2
    • Gaussian Upper : -5e-4
    • area upper: 50
    • area lower: 6
    • axratio lower: 0.5
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 3


  • PISA Param for 63x, 2048 x 2048
    • Gaussian Std : 3
    • Gaussian Upper : -2e-4
    • area upper: 81
    • area lower: 9
    • axratio lower: 0.6
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 4


  • PISA Param for 63x Zoom 4, 2048 x 2048
    • Gaussian Std : 5
    • Gaussian Upper : -2e-5
    • area upper: 1296
    • area lower: 81
    • axratio lower: 0.5
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 4