Hosuk:LabNotes/2014-7-18: Difference between revisions

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>Hosuki78
(Created page with "*LabNote ===Deconvolution and PSF images=== ====PSF Functions==== *Generated by '''PSF Generator''' plug-in **Settings [[File:PSFGeneratorSettings_2014-0...")
 
>Hosuki78
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**[img_Decv, PSF] = deconvblind(img_raw, img_PSF);
**[img_Decv, PSF] = deconvblind(img_raw, img_PSF);
*Then do medfilt2, and make img_double
*Then do medfilt2, and make img_double
**The latest Matlab code : [[Media:PISA7_Shield_Core_v2.m|'''PISA7_Shield_Core_v2.m''']]
**The latest Matlab code : [[Media:PISA7_Shield_Core_v3.m|'''PISA7_Shield_Core_v3.m''']]
 
 
 
===Update PISA Parameter===
*PISA Param for 20x, 2048 x 2048
**Gaussian Std : 2
**Gaussian Upper : -2e-4
**area upper: 16
**area lower: 3
**axratio lower: 0.5
**circ upper: 1.6
**circ lower: 0.8
**perim conn: 8
**bkgmult lower: 4
 
 
 
*PISA Param for 20x, 4096 x 4096
**Gaussian Std : 2
**Gaussian Upper : -5e-4
**area upper: 50
**area lower: 6
**axratio lower: 0.5
**circ upper: 1.6
**circ lower: 0.8
**perim conn: 8
**bkgmult lower: 3
 
 
 
*PISA Param for 63x, 2048 x 2048
**Gaussian Std : 3
**Gaussian Upper : -2e-4
**area upper: 81
**area lower: 9
**axratio lower: 0.6
**circ upper: 1.6
**circ lower: 0.8
**perim conn: 8
**bkgmult lower: 4
 
 
 
*PISA Param for 63x Zoom 4, 2048 x 2048
**Gaussian Std : 5
**Gaussian Upper : -2e-5
**area upper: 1296
**area lower: 81
**axratio lower: 0.5
**circ upper: 1.6
**circ lower: 0.8
**perim conn: 8
**bkgmult lower: 4

Latest revision as of 19:43, 1 September 2014

Deconvolution and PSF images[edit]

PSF Functions[edit]

  • Generated by PSF Generator plug-in
    • Settings

File:PSFGeneratorSettings 2014-07-18.png.png



Deconvolution[edit]

  • Use deconvblind function
    • [img_Decv, PSF] = deconvblind(img_raw, img_PSF);
  • Then do medfilt2, and make img_double


Update PISA Parameter[edit]

  • PISA Param for 20x, 2048 x 2048
    • Gaussian Std : 2
    • Gaussian Upper : -2e-4
    • area upper: 16
    • area lower: 3
    • axratio lower: 0.5
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 4


  • PISA Param for 20x, 4096 x 4096
    • Gaussian Std : 2
    • Gaussian Upper : -5e-4
    • area upper: 50
    • area lower: 6
    • axratio lower: 0.5
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 3


  • PISA Param for 63x, 2048 x 2048
    • Gaussian Std : 3
    • Gaussian Upper : -2e-4
    • area upper: 81
    • area lower: 9
    • axratio lower: 0.6
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 4


  • PISA Param for 63x Zoom 4, 2048 x 2048
    • Gaussian Std : 5
    • Gaussian Upper : -2e-5
    • area upper: 1296
    • area lower: 81
    • axratio lower: 0.5
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 4