Hosuk:LabNotes/2014-7-18: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===Deconvolution and PSF images=== ====PSF Functions==== *Generated by '''PSF Generator''' plug-in **Settings [[File:PSFGeneratorSettings_2014-0...") |
>Hosuki78 No edit summary |
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**[img_Decv, PSF] = deconvblind(img_raw, img_PSF); | **[img_Decv, PSF] = deconvblind(img_raw, img_PSF); | ||
*Then do medfilt2, and make img_double | *Then do medfilt2, and make img_double | ||
**The latest Matlab code : [[Media: | **The latest Matlab code : [[Media:PISA7_Shield_Core_v3.m|'''PISA7_Shield_Core_v3.m''']] | ||
===Update PISA Parameter=== | |||
*PISA Param for 20x, 2048 x 2048 | |||
**Gaussian Std : 2 | |||
**Gaussian Upper : -2e-4 | |||
**area upper: 16 | |||
**area lower: 3 | |||
**axratio lower: 0.5 | |||
**circ upper: 1.6 | |||
**circ lower: 0.8 | |||
**perim conn: 8 | |||
**bkgmult lower: 4 | |||
*PISA Param for 20x, 4096 x 4096 | |||
**Gaussian Std : 2 | |||
**Gaussian Upper : -5e-4 | |||
**area upper: 50 | |||
**area lower: 6 | |||
**axratio lower: 0.5 | |||
**circ upper: 1.6 | |||
**circ lower: 0.8 | |||
**perim conn: 8 | |||
**bkgmult lower: 3 | |||
*PISA Param for 63x, 2048 x 2048 | |||
**Gaussian Std : 3 | |||
**Gaussian Upper : -2e-4 | |||
**area upper: 81 | |||
**area lower: 9 | |||
**axratio lower: 0.6 | |||
**circ upper: 1.6 | |||
**circ lower: 0.8 | |||
**perim conn: 8 | |||
**bkgmult lower: 4 | |||
*PISA Param for 63x Zoom 4, 2048 x 2048 | |||
**Gaussian Std : 5 | |||
**Gaussian Upper : -2e-5 | |||
**area upper: 1296 | |||
**area lower: 81 | |||
**axratio lower: 0.5 | |||
**circ upper: 1.6 | |||
**circ lower: 0.8 | |||
**perim conn: 8 | |||
**bkgmult lower: 4 |
Latest revision as of 19:43, 1 September 2014
Deconvolution and PSF images[edit]
PSF Functions[edit]
- Generated by PSF Generator plug-in
- Settings
File:PSFGeneratorSettings 2014-07-18.png.png
- PSF_20x_4096x4096_best1st.tif
- PSF_63x_2048x2048_best1st.tif
- PSF_63xZoom4_2048x2048_best1st.tif
- Each z0 images were selected from the result of the stack of PSF images.
Deconvolution[edit]
- Use deconvblind function
- [img_Decv, PSF] = deconvblind(img_raw, img_PSF);
- Then do medfilt2, and make img_double
- The latest Matlab code : PISA7_Shield_Core_v3.m
Update PISA Parameter[edit]
- PISA Param for 20x, 2048 x 2048
- Gaussian Std : 2
- Gaussian Upper : -2e-4
- area upper: 16
- area lower: 3
- axratio lower: 0.5
- circ upper: 1.6
- circ lower: 0.8
- perim conn: 8
- bkgmult lower: 4
- PISA Param for 20x, 4096 x 4096
- Gaussian Std : 2
- Gaussian Upper : -5e-4
- area upper: 50
- area lower: 6
- axratio lower: 0.5
- circ upper: 1.6
- circ lower: 0.8
- perim conn: 8
- bkgmult lower: 3
- PISA Param for 63x, 2048 x 2048
- Gaussian Std : 3
- Gaussian Upper : -2e-4
- area upper: 81
- area lower: 9
- axratio lower: 0.6
- circ upper: 1.6
- circ lower: 0.8
- perim conn: 8
- bkgmult lower: 4
- PISA Param for 63x Zoom 4, 2048 x 2048
- Gaussian Std : 5
- Gaussian Upper : -2e-5
- area upper: 1296
- area lower: 81
- axratio lower: 0.5
- circ upper: 1.6
- circ lower: 0.8
- perim conn: 8
- bkgmult lower: 4