Daniel:Notebook/HiResChrPaint/2014-7-18: Difference between revisions
Jump to navigation
Jump to search
>Djacobse (Created page with "=Dystrophin Probes= 2014-7-18 The dystrophin probes I designed last week arrived from IDT. The...") |
>Djacobse No edit summary |
||
Line 3: | Line 3: | ||
[[Daniel:Notebook/HiResChrPaint|2014-7-18]] | [[Daniel:Notebook/HiResChrPaint|2014-7-18]] | ||
The dystrophin probes I designed [[Daniel:Notebook/HiResChrPaint/2014-7-10|last week]] arrived from IDT. The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file: | The dystrophin probes I designed [[Daniel:Notebook/HiResChrPaint/2014-7-10|last week]] arrived from IDT. Attached are their specs: | ||
[[Media:IDT_PlateSpecs_IDTOrder.txt|IDT Order Specs]] | |||
The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file: | |||
*[[Media:IDT_PlateSpecs_07172014.txt|IDT Plate Spec Calculations]] | *[[Media:IDT_PlateSpecs_07172014.txt|IDT Plate Spec Calculations]] |
Revision as of 19:42, 18 July 2014
Dystrophin Probes
The dystrophin probes I designed last week arrived from IDT. Attached are their specs:
The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file:
Oligo Pool Synthesis
- Resuspend each oligo to make them all 100 uM (See Plate Specs for values)
- Combine 20 uL from each oligo, makes 2 ug/uL total pool
- Add 640 uL nfH2O, makes 1 ug/uL total pool