Daniel:Notebook/HiResChrPaint/2014-7-18: Difference between revisions

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The dystrophin probes I designed [[Daniel:Notebook/HiResChrPaint/2014-7-10|last week]] arrived from IDT.  The oligos arrived as dry oligos in a plate.  The calculations I made are in the following text file:
The dystrophin probes I designed [[Daniel:Notebook/HiResChrPaint/2014-7-10|last week]] arrived from IDT.  Attached are their specs:
 
[[Media:IDT_PlateSpecs_IDTOrder.txt|IDT Order Specs]]
 
The oligos arrived as dry oligos in a plate.  The calculations I made are in the following text file:


*[[Media:IDT_PlateSpecs_07172014.txt|IDT Plate Spec Calculations]]
*[[Media:IDT_PlateSpecs_07172014.txt|IDT Plate Spec Calculations]]

Revision as of 19:42, 18 July 2014

Dystrophin Probes

2014-7-18

The dystrophin probes I designed last week arrived from IDT. Attached are their specs:

IDT Order Specs

The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file:

Oligo Pool Synthesis

  1. Resuspend each oligo to make them all 100 uM (See Plate Specs for values)
  2. Combine 20 uL from each oligo, makes 2 ug/uL total pool
  3. Add 640 uL nfH2O, makes 1 ug/uL total pool