Daniel:Notebook/HiResChrPaint/2014-7-18: Difference between revisions
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The dystrophin probes I designed [[Daniel:Notebook/HiResChrPaint/2014-7-10|last week]] arrived from IDT. Attached are their specs: | The dystrophin probes I designed [[Daniel:Notebook/HiResChrPaint/2014-7-10|last week]] arrived from IDT. Attached are their specs: | ||
[[Media:IDT_PlateSpecs_IDTOrder.txt|IDT Order Specs]] | *[[Media:IDT_PlateSpecs_IDTOrder.txt|IDT Order Specs]] | ||
The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file: | The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file: |
Revision as of 19:43, 18 July 2014
Dystrophin Probes
The dystrophin probes I designed last week arrived from IDT. Attached are their specs:
The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file:
Oligo Pool Synthesis
- Resuspend each oligo to make them all 100 uM (See Plate Specs for values)
- Combine 20 uL from each oligo, makes 2 ug/uL total pool
- Add 640 uL nfH2O, makes 1 ug/uL total pool