Daniel:Notebook/HiResChrPaint/2014-7-18: Difference between revisions
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==Oligo Pool Synthesis== | ==Oligo Pool Synthesis== | ||
#Resuspend each oligo to make them all | #Resuspend each oligo to make them all 1 mM (See Plate Specs for values) | ||
#Combine | #Combine 2 uL from each oligo, makes 12.5 ug/uL total pool | ||
#Add | ##Total volume will be 64 uL | ||
#Add 742.4 uL nfH2O, makes 1 ug/uL total pool |
Revision as of 20:25, 18 July 2014
Dystrophin Probes
The dystrophin probes I designed last week arrived from IDT. Attached are their specs:
The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file:
Oligo Pool Synthesis
- Resuspend each oligo to make them all 1 mM (See Plate Specs for values)
- Combine 2 uL from each oligo, makes 12.5 ug/uL total pool
- Total volume will be 64 uL
- Add 742.4 uL nfH2O, makes 1 ug/uL total pool