Daniel:Notebook/HiResChrPaint/2014-7-18: Difference between revisions

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==Oligo Pool Synthesis==
==Oligo Pool Synthesis==


#Resuspend each oligo to make them all 100 uM (See Plate Specs for values)
#Resuspend each oligo to make them all 1 mM (See Plate Specs for values)
#Combine 20 uL from each oligo, makes 2 ug/uL total pool
#Combine 2 uL from each oligo, makes 12.5 ug/uL total pool
#Add 640 uL nfH2O, makes 1 ug/uL total pool
##Total volume will be 64 uL
#Add 742.4 uL nfH2O, makes 1 ug/uL total pool

Revision as of 20:25, 18 July 2014

Dystrophin Probes

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The dystrophin probes I designed last week arrived from IDT. Attached are their specs:

The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file:

Oligo Pool Synthesis

  1. Resuspend each oligo to make them all 1 mM (See Plate Specs for values)
  2. Combine 2 uL from each oligo, makes 12.5 ug/uL total pool
    1. Total volume will be 64 uL
  3. Add 742.4 uL nfH2O, makes 1 ug/uL total pool