Daniel:Notebook/HiResChrPaint/2014-7-18: Difference between revisions
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=Dystrophin Probes= | =Dystrophin Probes= | ||
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The dystrophin probes I designed [[Daniel:Notebook/HiResChrPaint/2014-7-10|last week]] arrived from IDT. Attached are their specs: | The dystrophin probes I designed [[Daniel:Notebook/HiResChrPaint/2014-7-10|last week]] arrived from IDT. Attached are their specs: |
Revision as of 19:49, 19 July 2014
Dystrophin Probes
The dystrophin probes I designed last week arrived from IDT. Attached are their specs:
The oligos arrived as dry oligos in a plate. The calculations I made are in the following text file:
Oligo Pool Synthesis
- Resuspend each oligo to make them all 1 mM (See Plate Specs for values)
- Combine 2 uL from each oligo
- Makes 12.5 ug/uL total pool
- Total volume will be 64 uL
- Add 742.4 uL nfH2O, dilutes to 1 ug/uL total pool