Blue:RNA-Seq Experiments:07282014: Difference between revisions

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>B1lake
(Created page with "==SmartPlus on Mouse Nuclei== Obtained sorted nuclei from Aran: #100 nuclei sorted directly into 2ul clontech dilution buffer (4x) #500 nuclei sorted directly into 2ul clonte...")
 
>B1lake
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*RNA extraction of 5000 using Zymo Micro RNA Kit (including DNAse treatment). Eluted in 6ul and vacuum spun down to 2ul.
*RNA extraction of 5000 using Zymo Micro RNA Kit (including DNAse treatment). Eluted in 6ul and vacuum spun down to 2ul.


*Performed SmartPlus protocol as per C1 tube control protocol on 1ul of above samples (only modification is addition of randomer)
*Performed SmartPlus protocol as per C1 tube control protocol on 1ul of above samples (only modification is addition of randomer) using 21 cycles for PCR

Revision as of 18:34, 29 July 2014

SmartPlus on Mouse Nuclei

Obtained sorted nuclei from Aran:

  1. 100 nuclei sorted directly into 2ul clontech dilution buffer (4x)
  2. 500 nuclei sorted directly into 2ul clontech dilution buffer (4x)
  3. 5000 nuclei sorted into BSA buffer (2x)
  • stored 100/500 sorts at -80C. Vacuum spun down to 1ul for each.
  • RNA extraction of 5000 using Zymo Micro RNA Kit (including DNAse treatment). Eluted in 6ul and vacuum spun down to 2ul.
  • Performed SmartPlus protocol as per C1 tube control protocol on 1ul of above samples (only modification is addition of randomer) using 21 cycles for PCR