Blue:RNA-Seq Experiments:07282014: Difference between revisions
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>B1lake (Created page with "==SmartPlus on Mouse Nuclei== Obtained sorted nuclei from Aran: #100 nuclei sorted directly into 2ul clontech dilution buffer (4x) #500 nuclei sorted directly into 2ul clonte...") |
>B1lake |
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*RNA extraction of 5000 using Zymo Micro RNA Kit (including DNAse treatment). Eluted in 6ul and vacuum spun down to 2ul. | *RNA extraction of 5000 using Zymo Micro RNA Kit (including DNAse treatment). Eluted in 6ul and vacuum spun down to 2ul. | ||
*Performed SmartPlus protocol as per C1 tube control protocol on 1ul of above samples (only modification is addition of randomer) | *Performed SmartPlus protocol as per C1 tube control protocol on 1ul of above samples (only modification is addition of randomer) using 21 cycles for PCR |
Revision as of 18:34, 29 July 2014
SmartPlus on Mouse Nuclei
Obtained sorted nuclei from Aran:
- 100 nuclei sorted directly into 2ul clontech dilution buffer (4x)
- 500 nuclei sorted directly into 2ul clontech dilution buffer (4x)
- 5000 nuclei sorted into BSA buffer (2x)
- stored 100/500 sorts at -80C. Vacuum spun down to 1ul for each.
- RNA extraction of 5000 using Zymo Micro RNA Kit (including DNAse treatment). Eluted in 6ul and vacuum spun down to 2ul.
- Performed SmartPlus protocol as per C1 tube control protocol on 1ul of above samples (only modification is addition of randomer) using 21 cycles for PCR