Hosuk:LabNotes/2014-7-31: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===Decoding for Mutation detection of Cancer Cell===") |
>Hosuki78 No edit summary |
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===Decoding for Mutation detection of Cancer Cell=== | ===Decoding for Mutation detection of Cancer Cell=== | ||
*Try decoding (07/30, 07/31) | |||
*With Two samples | |||
**S1_0715 : +Ampligase and +KlenTaq at 45C for 24hr. | |||
**S2_0715 : +Ampligase and +KlenTaq at 60C for 24hr. | |||
====Result==== | |||
*No signal… need to check again! | |||
*1st Rolony signal was not detected. | |||
*Another sample, ampligase at 60C : also no signal, somehow 1st rolonies were gone… | |||
*I guess 1st Rolonies were unfold and gone somehow. | |||
*will try again. |
Revision as of 18:50, 31 July 2014
Decoding for Mutation detection of Cancer Cell
- Try decoding (07/30, 07/31)
- With Two samples
- S1_0715 : +Ampligase and +KlenTaq at 45C for 24hr.
- S2_0715 : +Ampligase and +KlenTaq at 60C for 24hr.
Result
- No signal… need to check again!
- 1st Rolony signal was not detected.
- Another sample, ampligase at 60C : also no signal, somehow 1st rolonies were gone…
- I guess 1st Rolonies were unfold and gone somehow.
- will try again.