Matt:LabNotes/2014-8-4: Difference between revisions

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>Mzcai
(Created page with "==Agi26k0gap Probe Production Continued== *Continued from: Matt:LabNotes/2014-7-22 **Thaw and combine the 7 tubes of 200ul that were stored at -20C ===TBU Gel Check=== *2...")
 
>Mzcai
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*Undigested sample: 10nM 1st round amplicon
*Undigested sample: 10nM 1st round amplicon


[[]]
[[File:2014-08-04_Agi26k0gapDigestCheck.jpg]]
*Brightest band below 200bp as expected
*Brightest band below 200bp as expected


===Column Purification===
===Column Purification===
*Used Zymo ssDNA/RNA columns
*Used Zymo ssDNA/RNA columns
*Total Eluted: ul
*Total Eluted: 124 ul


*Nanodrop quant:  ng/ul
*Nanodrop quant:  137.6 ng/ul
*Yield:  ng/ul x  ul = ug -->
*Yield:  137.6 ng/ul x  124 ul = 17.06 ug
 
*Continued on: [[Matt:LabNotes/2014-8-7]]

Latest revision as of 16:48, 7 August 2014

Agi26k0gap Probe Production Continued[edit]

TBU Gel Check[edit]

  • 2ul Sample + 2ul 2X Urea Loading Dye
  • 0.5ul Low Mass Ladder + 1.5ul H2O + 2ul 2X Urea Loading Dye
  • Digested sample
  • Undigested sample: 10nM 1st round amplicon

File:2014-08-04 Agi26k0gapDigestCheck.jpg

  • Brightest band below 200bp as expected

Column Purification[edit]

  • Used Zymo ssDNA/RNA columns
  • Total Eluted: 124 ul
  • Nanodrop quant: 137.6 ng/ul
  • Yield: 137.6 ng/ul x 124 ul = 17.06 ug