Matt:LabNotes/2014-8-4: Difference between revisions
Jump to navigation
Jump to search
>Mzcai (Created page with "==Agi26k0gap Probe Production Continued== *Continued from: Matt:LabNotes/2014-7-22 **Thaw and combine the 7 tubes of 200ul that were stored at -20C ===TBU Gel Check=== *2...") |
>Mzcai mNo edit summary |
||
(3 intermediate revisions by the same user not shown) | |||
Line 9: | Line 9: | ||
*Undigested sample: 10nM 1st round amplicon | *Undigested sample: 10nM 1st round amplicon | ||
[[]] | [[File:2014-08-04_Agi26k0gapDigestCheck.jpg]] | ||
*Brightest band below 200bp as expected | *Brightest band below 200bp as expected | ||
===Column Purification=== | ===Column Purification=== | ||
*Used Zymo ssDNA/RNA columns | *Used Zymo ssDNA/RNA columns | ||
*Total Eluted: ul | *Total Eluted: 124 ul | ||
*Nanodrop quant: ng/ul | *Nanodrop quant: 137.6 ng/ul | ||
*Yield: ng/ul x ul = | *Yield: 137.6 ng/ul x 124 ul = 17.06 ug | ||
*Continued on: [[Matt:LabNotes/2014-8-7]] |
Latest revision as of 16:48, 7 August 2014
Agi26k0gap Probe Production Continued[edit]
- Continued from: Matt:LabNotes/2014-7-22
- Thaw and combine the 7 tubes of 200ul that were stored at -20C
TBU Gel Check[edit]
- 2ul Sample + 2ul 2X Urea Loading Dye
- 0.5ul Low Mass Ladder + 1.5ul H2O + 2ul 2X Urea Loading Dye
- Digested sample
- Undigested sample: 10nM 1st round amplicon
File:2014-08-04 Agi26k0gapDigestCheck.jpg
- Brightest band below 200bp as expected
Column Purification[edit]
- Used Zymo ssDNA/RNA columns
- Total Eluted: 124 ul
- Nanodrop quant: 137.6 ng/ul
- Yield: 137.6 ng/ul x 124 ul = 17.06 ug
- Continued on: Matt:LabNotes/2014-8-7