Daniel:Notebook/HiResChrPaint/2014-8-6: Difference between revisions

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As expected, the background is definitely higher, but the signal is also stronger.  It will be interesting to do a wash on these samples, and then examine them after.  Given the success, I'm going to return the positive control to the incubator and I'll take snapshots of the other 3 samples.
As expected, the background is definitely higher, but the signal is also stronger.  It will be interesting to do a wash on these samples, and then examine them after.  Given the success, I'm going to return the positive control to the incubator and I'll take snapshots of the other 3 samples.
===Sample A===
Sample A is the Dystrophin gene labeled with Alexa 488.  The sample has not been washed yet, but was prepared in the same manner I normally prepare samples.
<gallery>
File:20140806_DMD-488_PreWash.lif_DMD488_1_ch00.jpg|Cells stained with DMD, Alexa 488 (green), field 1
File:20140806_DMD-488_PreWash.lif_DMD488_2_ch00.jpg|Cells stained with DMD, Alexa 488 (green), field 2
File:20140806_DMD-488_PreWash.lif_DMD488_3_ch00.jpg|Cells stained with DMD, Alexa 488 (green), field 3
File:20140806_DMD-488_PreWash.lif_DMD488_4_ch00.jpg|Cells stained with DMD, Alexa 488 (green), field 4
File:20140806_DMD-488_PreWash.lif_DMD488_5_ch00.jpg|Cells stained with DMD, Alexa 488 (green), field 5
File:20140806_DMD-488_PreWash.lif_DMD488_6_ch00.jpg|Cells stained with DMD, Alexa 488 (green), field 6
</gallery>
===Sample B===
Sample B is the Dystrophin gene labeled with Alexa 546.  The sample has not been washed yet, but was prepared in the same manner I normally prepare samples.
<gallery>
File:Experiment002_DMD546_1_ch00.jpg|Cells stained with DMD, Alexa 546 (red), field 1
File:Experiment002_DMD546_2_ch00.jpg|Cells stained with DMD, Alexa 546 (red), field 2
</gallery>

Revision as of 23:16, 6 August 2014

No Wash FISH

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Microscopy

First I'll test whether the samples can be taken directly to the microscope, with the hybridization buffer still on. This will produce a higher background noise, but will hopefully still result in strong areas of signal distinguishable from the background. Since this is just a test, I'll only use the positive control (sample D, Cot1-488) for now, and I'll investigate the others pending successful trial.

Sample D

As expected, the background is definitely higher, but the signal is also stronger. It will be interesting to do a wash on these samples, and then examine them after. Given the success, I'm going to return the positive control to the incubator and I'll take snapshots of the other 3 samples.

Sample A

Sample A is the Dystrophin gene labeled with Alexa 488. The sample has not been washed yet, but was prepared in the same manner I normally prepare samples.

Sample B

Sample B is the Dystrophin gene labeled with Alexa 546. The sample has not been washed yet, but was prepared in the same manner I normally prepare samples.