Daniel:Notebook/HiResChrPaint/2014-8-17: Difference between revisions
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So there is very little left. I'm going to do a bigger amplification [[Daniel:Notebook/HiResChrPaint/2014-8-18|tomorrow]], and I'll pool today's samples with tomorrow's before continuing with USER/DpnII. | So there is very little left. I'm going to do a bigger amplification [[Daniel:Notebook/HiResChrPaint/2014-8-18|tomorrow]], and I'll pool today's samples with tomorrow's before continuing with USER/DpnII. | ||
[[Category:HRCP]] [[Category:Lambda Exo]] [[Category:20140813]] |
Latest revision as of 22:38, 9 September 2014
Padlock Probes (Started 8/17/2014)[edit]
Lambda Exonuclease Digestion[edit]
Reaction Table
Reagent | uL |
Amplicon | 70 |
Lambda Exonuclease Buffer (10X) | 10 |
Lambda Exonuclease | 5 |
nf H2O | 15 |
Total | 100 |
- Set up reaction according to table
- Incubate at 37C for 1 hour
- Purify using 2 ssDNA columns, elute with 20 uL nfH2O
Results[edit]
Sample | ng/uL | ug in 20 uL | ug Total |
PPRL-XO-A | 10.5 | 0.2 | |
PPRL-XO-B | 20.8 | 0.4 | 0.6 |
So there is very little left. I'm going to do a bigger amplification tomorrow, and I'll pool today's samples with tomorrow's before continuing with USER/DpnII.