Sam:LabNotes/Microbione/2009-2-5: Difference between revisions
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>Sam Chiang |
>Sam Chiang |
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'''FIG.1 Realtime amplification plot (no log in flourescent)''' | '''FIG.1 Realtime amplification plot (no log in flourescent)''' | ||
[[Image:sam020409.jpg]] | [[Image:sam020409.jpg|frame]] | ||
'''FIG.2 Realtime amplification plot (with log in flourescent)''' | '''FIG.2 Realtime amplification plot (with log in flourescent)''' | ||
[[Image:sam020409-log.jpg]] | [[Image:sam020409-log.jpg|frame]] | ||
==Discussion== | ==Discussion== |
Revision as of 20:51, 5 February 2009
Follow up: Testing human 18S and Bac (E. coli) 16S primers using realtime PCR
Exp. Design
Template gDNA H2O -------- --- Primer: 18S-211 #1 #2 #3 (#1, #2 - replicates) 18S-306 #4 #5 #6 (#4, #5 - replicates)
Results
FIG.1 Realtime amplification plot (no log in flourescent)
FIG.2 Realtime amplification plot (with log in flourescent)
Discussion
- The amplification curves are not distinguishable (Sample vs. Blank) within 20 clycles.
- The amplifications cycless were not enough?
- The amplifications are not working?
- Primers' specificity? binding affinity?
- Concentration of templates?